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Overview

  • Product nameAnti-Smad1 antibodySee all Smad1 primary antibodies ...
  • Description
    Mouse monoclonal to Smad1
  • Tested applicationsWB, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-466 of Human Smad1

Properties

Applications

Our Abpromise guarantee covers the use of ab55476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 52 kDa.
IHC-P IHC-P: Use a concentration of 3 µg/ml.
ICC/IF ICC/IF: Use a concentration of 10 µg/ml.

Target

  • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.

    • Target information above from: UniProt accession Q15797 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        BSP-1 antibodyBSP1 antibodyHsMAD1 antibody
        JV4-1 antibodyJV41 antibodyMAD homolog 1 antibodyMAD mothers against decapentaplegic homolog 1 antibodyMad related protein 1 antibodyMad-related protein 1 antibodyMADH1 antibodyMADR1 antibodyMothers against decapentaplegic homolog 1 antibodyMothers against DPP homolog 1 antibodySMA- AND MAD-RELATED PROTEIN 1 antibodySMAD 1 antibodySMAD family member 1 antibodySMAD mothers against DPP homolog 1 antibodySmad1 antibodySmad1 antibodySMAD1_HUMAN antibodyTGF beta signaling protein 1 antibodyTransforming growth factor-beta-signaling protein 1 antibody
      see all

    Anti-Smad1 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Smad1 antibody (ab55476)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Smad1 antibody (ab55476)
      Smad1 antibody (ab55476) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
    • Western blot - Smad1 antibody (ab55476)
      Western blot - Smad1 antibody (ab55476)


      Predicted band size : 52 kDa
      Smad1 antibody (ab55476) at 1ug/lane + HeLa cell lysate at 25ug/lane.
    • Immunocytochemistry/ Immunofluorescence-Smad1 antibody(ab55476)
      Immunocytochemistry/ Immunofluorescence-Smad1 antibody(ab55476)
      ab55476 at 10 ug/ml staining smad1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

    References for Anti-Smad1 antibody (ab55476)

    ab55476 has not yet been referenced specifically in any publications.

    Publishing research using ab55476 ? Please let us know so that we can cite the reference in this datasheet

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