Purification notesab73211 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
Use at an assay dependent concentration.
FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
Sequence similaritiesBelongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain.
Post-translational modificationsPhosphorylated on serine by BMP type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
Immunohistochemical analysis of Smad1 in paraffin-embedded
human breast carcinoma tissue using ab73211 at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising phosphopeptide.
Western blot - Smad1 (phospho S187) antibody (ab73211)
All lanes : Anti-Smad1 (phospho S187) antibody (ab73211) at 1/500 dilution
Lane 1 : Mouse muscle cell extract Lane 2 : Mouse muscle cell extract with immunising phosphopeptide at 10 µg
Immunohistochemistry (Frozen sections) - Anti-Smad1 (phospho S187) antibody (ab73211)This image is courtesy of an anonymous Abreview
ab73211 staining Smad1 (phospho S187) in Human skin, hair follicle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. A TRITC-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.