Anti-Smad1 (phospho S187) antibody (ab73211)

Overview

• Product nameAnti-Smad1 (phospho S187) antibodySee all Smad1 primary antibodies ...
• Description
  Rabbit polyclonal to Smad1 (phospho S187)
• Specificityab73211 detects endogenous levels of Smad1 only when phosphorylated at serine 187.
• Tested applicationsWB, ELISA, IHC-P, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of serine 187 (PHS^PPN)

• Positive controlHuman breast carcinoma tissue. Mouse muscle cell extract.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab73211 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Cancer
  • Growth factors
  • TGF

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • SMADs

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • SMADs

Applications

Target

• FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
• Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
• Sequence similaritiesBelongs to the dwarfin/SMAD family.
  Contains 1 MH1 (MAD homology 1) domain.
  Contains 1 MH2 (MAD homology 2) domain.
• Post-translational
  modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
  Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
• Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.

Target information above from: UniProt accession Q15797 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4086 Human
  • Entrez Gene: 17125 Mouse
  • Omim: 601595 Human
  • SwissProt: Q15797 Human
  • SwissProt: P70340 Mouse
  • Unigene: 604588 Human
  • Unigene: 223717 Mouse

• Alternative names
  BSP 1 antibodyBSP-1 antibodyBSP1 antibody

  Dwarfin A antibodyDwfA antibodyhSMAD 1 antibodyhSMAD1 antibodyJV 41 antibodyJV4 1 antibodyJV4-1 antibodyJV41 antibodyMAD homolog 1 antibodyMAD mothers against decapentaplegic homolog 1 antibodyMad related protein 1 antibodyMad-related protein 1 antibodyMADH 1 antibodyMADH1 antibodyMadr 1 antibodyMADR1 antibodyMothers against decapentaplegic homolog 1 antibodyMothers against DPP homolog 1 antibodySma and Mad related protein 1 antibodySMA- AND MAD-RELATED PROTEIN 1 antibodySMAD 1 antibodySMAD family member 1 antibodySMAD mothers against DPP homolog 1 antibodySMAD mothers against DPP homolog 1 Drosophila antibodySmad1 antibodySmad1 antibodySMAD1_HUMAN antibodySpinal muscular atrophy distal with upper limb predominance antibodyTGF beta signaling protein 1 antibodyTransforming growth factor beta signaling protein 1 antibodyTransforming growth factor-beta-signaling protein 1 antibody

  see all

Anti-Smad1 (phospho S187) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Smad1 (phospho S187) antibody (ab73211)
  Immunohistochemical analysis of Smad1 in paraffin-embedded human breast carcinoma tissue using ab73211 at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising phosphopeptide.
• 

  Western blot - Smad1 (phospho S187) antibody (ab73211)
  All lanes : Anti-Smad1 (phospho S187) antibody (ab73211) at 1/500 dilution
  
  Lane 1 : Mouse muscle cell extract
  Lane 2 : Mouse muscle cell extract with immunising phosphopeptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 52 kDa
  Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Frozen sections) - Anti-Smad1 (phospho S187) antibody (ab73211)This image is courtesy of an anonymous Abreview
  ab73211 staining Smad1 (phospho S187) in Human skin, hair follicle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. A TRITC-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

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