Anti-Smad1 (phospho S465) antibody (ab63439)

Overview

  • Product nameAnti-Smad1 (phospho S465) antibody
    See all Smad1 primary antibodies
  • Description
    Rabbit polyclonal to Smad1 (phospho S465)
  • Specificityab63439 detects endogenous levels of Smad1 only when phosphorylated at serine 465.
  • Tested applicationsELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of serine 465 (ISSVSP).

  • Positive control
    • Human breast carcinoma tissue and HeLa cell extracts treated with Serum (10%, 15min).

Properties

Applications

Our Abpromise guarantee covers the use of ab63439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BSP-1 antibody
    • BSP1 antibody
    • HsMAD1 antibody
    • JV4-1 antibody
    • JV41 antibody
    • MAD homolog 1 antibody
    • MAD mothers against decapentaplegic homolog 1 antibody
    • Mad related protein 1 antibody
    • Mad-related protein 1 antibody
    • MADH1 antibody
    • MADR1 antibody
    • Mothers against decapentaplegic homolog 1 antibody
    • Mothers against DPP homolog 1 antibody
    • SMA- AND MAD-RELATED PROTEIN 1 antibody
    • SMAD 1 antibody
    • SMAD family member 1 antibody
    • SMAD mothers against DPP homolog 1 antibody
    • Smad1 antibody
    • Smad1 antibody
    • SMAD1_HUMAN antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor-beta-signaling protein 1 antibody
    see all

Anti-Smad1 (phospho S465) antibody images

  • All lanes : Anti-Smad1 (phospho S465) antibody (ab63439) at 1/500 dilution

    Lane 1 : HeLa cell extracts treated with Serum (10%, 15min)
    Lane 2 : HeLa cell extracts treated with Serum (10%, 15min) with immunising peptide at 10 µg


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ab63439 at 1/50 - 1/100 dilution staining Smad1 in human breast carcinomaby Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-Smad1 (phospho S465) antibody (ab63439)

This product has been referenced in:
  • Zhen G  et al. Inhibition of TGF-ß signaling in mesenchymal stem cells of subchondral bone attenuates osteoarthritis. Nat Med 19:704-12 (2013). Read more (PubMed: 23685840) »
  • Leeper NJ  et al. MicroRNA-26a is a novel regulator of vascular smooth muscle cell function. J Cell Physiol 226:1035-43 (2011). WB ; Human . Read more (PubMed: 20857419) »

See all 2 Publications for this product

Product Wall

I have contacted the lab who have agreed to add this product to the catalogue. The product has been added under catalogue number: ab183567, Smad1 peptide.

Thank you for contacting us.

We cannot disclose our exact immunogen sequence of ab63439. However, the amino acid range of this immunogen is aa415-465 of human Mothers against decapentaplegic homolog 1 with ACC#Q15797 (http://www.uniprot.org/...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (Imaginal wing disc)
Specification Imaginal wing disc
Fixative Formaldehyde
Permeabilization Yes - 0.1% TritonX-100
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Jul 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (hESC)
Loading amount 20 µg
Specification hESC
Treatment BMP4 for 24h
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"