Anti-Smad1 (phospho S465) antibody (ab97689)

Overview

  • Product name
    Anti-Smad1 (phospho S465) antibody
    See all Smad1 primary antibodies
  • Description
    Rabbit polyclonal to Smad1 (phospho S465)
  • Specificity
    ab97689 detects endogenous levels of Smad1 only when phosphorylated at serine 465.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from Human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).

  • Positive control
    • HeLa cell extracts treated with PMA.

Properties

Applications

Our Abpromise guarantee covers the use of ab97689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).

Target

  • Function
    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • Tissue specificity
    Ubiquitous. Highest expression seen in the heart and skeletal muscle.
  • Sequence similarities
    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on serine by BMP type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BSP-1 antibody
    • BSP1 antibody
    • HsMAD1 antibody
    • JV4-1 antibody
    • JV41 antibody
    • MAD homolog 1 antibody
    • MAD mothers against decapentaplegic homolog 1 antibody
    • Mad related protein 1 antibody
    • Mad-related protein 1 antibody
    • MADH1 antibody
    • MADR1 antibody
    • Mothers against decapentaplegic homolog 1 antibody
    • Mothers against DPP homolog 1 antibody
    • SMA- AND MAD-RELATED PROTEIN 1 antibody
    • SMAD 1 antibody
    • SMAD family member 1 antibody
    • SMAD mothers against DPP homolog 1 antibody
    • Smad1 antibody
    • SMAD1_HUMAN antibody
    • TGF beta signaling protein 1 antibody
    • Transforming growth factor-beta-signaling protein 1 antibody
    see all

Images

  • All lanes : Anti-Smad1 (phospho S465) antibody (ab97689) at 1/500 dilution

    Lane 1 : HeLa cell extracts, untreated
    Lane 2 : HeLa cell extracts, treated with PMA


    Predicted band size : 52 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (st.10.5 embryo)
Permeabilization
No
Specification
st.10.5 embryo
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 20 2016

Thank you for contacting us.

We cannot disclose our exact immunogen sequence of ab63439. However, the amino acid range of this immunogen is aa415-465 of human Mothers against decapentaplegic homolog 1 with ACC#Q15797 (http://www.uniprot.org/...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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