Anti-Smad1 (phospho S465) antibody (ab97689)

Overview

• Product nameAnti-Smad1 (phospho S465) antibodySee all Smad1 primary antibodies ...
• Description
  Rabbit polyclonal to Smad1 (phospho S465)
• Specificityab97689 detects endogenous levels of Smad1 only when phosphorylated at serine 465.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic phosphopeptide derived from Human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-S^P).

• Positive controlHeLa cell extracts treated with PMA.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab97689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Cancer
  • Growth factors
  • TGF

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • SMADs

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • SMADs

Applications

Target

• FunctionTranscriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
• Tissue specificityUbiquitous. Highest expression seen in the heart and skeletal muscle.
• Sequence similaritiesBelongs to the dwarfin/SMAD family.
  Contains 1 MH1 (MAD homology 1) domain.
  Contains 1 MH2 (MAD homology 2) domain.
• Post-translational
  modificationsPhosphorylated on serine by BMP type 1 receptor kinase.
  Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
• Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.

Target information above from: UniProt accession Q15797 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4086 Human
  • Entrez Gene: 17125 Mouse
  • Entrez Gene: 25671 Rat
  • Omim: 601595 Human
  • SwissProt: Q15797 Human
  • SwissProt: P70340 Mouse
  • SwissProt: P97588 Rat
  • Unigene: 604588 Human

  • Unigene: 223717 Mouse
  • Unigene: 10635 Rat

  see all

• Alternative names
  BSP-1 antibodyBSP1 antibodyHsMAD1 antibody

  JV4-1 antibodyJV41 antibodyMAD homolog 1 antibodyMAD mothers against decapentaplegic homolog 1 antibodyMad related protein 1 antibodyMad-related protein 1 antibodyMADH1 antibodyMADR1 antibodyMothers against decapentaplegic homolog 1 antibodyMothers against DPP homolog 1 antibodySMA- AND MAD-RELATED PROTEIN 1 antibodySMAD 1 antibodySMAD family member 1 antibodySMAD mothers against DPP homolog 1 antibodySmad1 antibodySmad1 antibodySMAD1_HUMAN antibodyTGF beta signaling protein 1 antibodyTransforming growth factor-beta-signaling protein 1 antibody

  see all

Anti-Smad1 (phospho S465) antibody images

• 

  Western blot - Smad1 (phospho S465) antibody (ab97689)
  All lanes : Anti-Smad1 (phospho S465) antibody (ab97689) at 1/500 dilution
  
  Lane 1 : HeLa cell extracts, untreated
  Lane 2 : HeLa cell extracts, treated with PMA
  
  
  Predicted band size : 52 kDa