The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 48 kDa.
RelevanceSMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
Cellular localizationCytoplasm. Nucleus.
Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
All lanes : Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution
Lane 1 : COS7 cell extracts treated with UV (15mins) Lane 2 : COS7 cell extracts untreated Lane 3 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) Lane 4 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg