• Product nameAnti-Smad2 + Smad3 (phospho T8) antibody
    See all Smad2 + Smad3 primary antibodies
  • Description
    Rabbit polyclonal to Smad2 + Smad3 (phospho T8)
  • SpecificityThis antibody detects endogenous levels of Smad2 + Smad3 only when phosphorylated at threonine 8.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthesized phosphopeptide derived from human Smad2+Smad3 around the phosphorylation site of threonine 8 (PFTPPP)

  • Positive control
    • Extracts from RAW264.7 cells



Our Abpromise guarantee covers the use of ab63399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa.
ELISA 1/20000.


  • RelevanceSMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
  • Cellular localizationCytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • Database links
  • Alternative names
    • hMAD 2 antibody
    • hMAD 3 antibody
    • hSMAD2 antibody
    • hSMAD3 antibody
    • Mad related protein 2 antibody
    • MADH2 antibody
    • MADH3 antibody
    • MADR2 antibody
    • Mothers against DPP homolog 2 antibody
    • Mothers against DPP homolog 3 antibody
    • Sma and Mad related protein 2 antibody
    • SMA and MAD related protein 3 antibody
    • SMAD 2 antibody
    • SMAD 3 antibody
    • SMAD family member 2 antibody
    • SMAD family member 3 antibody
    • smad2+3 antibody
    • smad2/3 antibody
    see all

Anti-Smad2 + Smad3 (phospho T8) antibody images

  • All lanes : Anti-Smad2 + Smad3 (phospho T8) antibody (ab63399) at 1/500 dilution

    Lane 1 : Extracts from RAW264.7 cells
    Lane 2 : Extracts from RAW264.7 cells plus phospho peptide

    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.

References for Anti-Smad2 + Smad3 (phospho T8) antibody (ab63399)

This product has been referenced in:
  • Wei MG  et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-ß/Smad Signaling. Evid Based Complement Alternat Med 2016:5031890 (2016). WB ; Rat . Read more (PubMed: 27403197) »
  • Cho JH  et al. Alpha1-Antitrypsin Attenuates Renal Fibrosis by Inhibiting TGF-ß1-Induced Epithelial Mesenchymal Transition. PLoS One 11:e0162186 (2016). Read more (PubMed: 27607429) »

See all 5 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab63399.
Please use the links above to contact us or submit feedback about this product.