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Overview

  • Product nameAnti-Smad2 antibody [7A5]See all Smad2 primary antibodies ...
  • Description
    Mouse monoclonal [7A5] to Smad2
  • Tested applicationsWB, IP, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length human Smad2 protein purified from E.coli with His tag.

  • Positive controlHeLa, A431, NIH3T3 and PC12 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    IP: Use at 2.5µl/ml of lysate.
    WB: 1/2000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
    • Tissue specificityExpressed at high levels in skeletal muscle, heart and placenta.
    • Sequence similaritiesBelongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.
    • Post-translational
      modifications      Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
      In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
      Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.

      • Target information above from: UniProt accession Q15796 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        hMAD 2 antibodyhMAD-2 antibodyhSMAD2 antibody
        JV18 1 antibodyJV18 antibodyJV18-1 antibodyJV181 antibodyMAD antibodyMAD homolog 2 antibodyMAD Related Protein 2 antibodyMad-related protein 2 antibodyMADH2 antibodyMADR2 antibodyMGC22139 antibodyMGC34440 antibodyMothers Against Decapentaplegic Homolog 2 antibodymothers against DPP homolog 2 antibodyOTTHUMP00000163489 antibodySma and Mad related protein 2 antibodySMAD 2 antibodySMAD antibodySMAD family member 2 antibodySMAD, mothers against DPP homolog 2 antibodySMAD2 antibodySMAD2_HUMAN antibody
      see all

    Anti-Smad2 antibody [7A5] images

    • Western blot - Smad2 antibody [7A5] (ab71109)
      Western blot - Smad2 antibody [7A5] (ab71109)
      All lanes : Anti-Smad2 antibody [7A5] (ab71109) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : NIH3T3 cell lysate
      Lane 4 : PC12 cell lysate


      Predicted band size : 58 kDa
      Observed band size : 58 kDa
    • Western blot - Anti-Smad2 antibody [7A5] (ab71109)
      Western blot - Anti-Smad2 antibody [7A5] (ab71109)
      Anti-Smad2 antibody [7A5] (ab71109) at 1/2000 dilution + Smad2 protein (Tagged) (ab63176) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    References for Anti-Smad2 antibody [7A5] (ab71109)

    ab71109 has not yet been referenced specifically in any publications.

    Publishing research using ab71109 ? Please let us know so that we can cite the reference in this datasheet

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    Immunoprecipitation abreview for Anti-Smad2 antibody [7A5]

     Excellent
    Abreviews
    Application Immunoprecipitation
    Sample Mouse Cell lysate - whole cell (lung epithelium)
    Total protein in input 600 µg
    Specification lung epithelium
    Immuno-precipitation step Protein A/G
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    Submitted May 18 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"