Anti-Smad2 antibody [EP567Y] (ab33875)

Overview

• Product nameAnti-Smad2 antibody [EP567Y]See all Smad2 primary antibodies ...
• Description
  Rabbit monoclonal [EP567Y] to Smad2
• SpecificityThis antibody detects a region about 40AA before the MH2 region(not the MH2 region itself)
• Tested applicationsWB, IHC-P, ICC, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A synthetic peptide corresponding to residues surrounding the MH2 domain of human Smad2.

• Positive controlJurkat cell lysate and human adenocarcinoma of uterus
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• Clonality Monoclonal
• Clone numberEP567Y
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Cancer
  • Growth factors
  • TGF

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • SMADs

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • SMADs

Applications

Target

• FunctionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
• Tissue specificityExpressed at high levels in skeletal muscle, heart and placenta.
• Sequence similaritiesBelongs to the dwarfin/SMAD family.
  Contains 1 MH1 (MAD homology 1) domain.
  Contains 1 MH2 (MAD homology 2) domain.
• Post-translational
  modificationsPhosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
  In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
  Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
• Cellular localizationCytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.

Target information above from: UniProt accession Q15796 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4087 Human
  • Entrez Gene: 17126 Mouse
  • Entrez Gene: 29357 Rat
  • Omim: 601366 Human
  • SwissProt: Q15796 Human
  • SwissProt: Q62432 Mouse
  • SwissProt: O70436 Rat
  • Unigene: 12253 Human

  • Unigene: 705764 Human
  • Unigene: 391091 Mouse
  • Unigene: 2755 Rat

  see all

• Alternative names
  hMAD 2 antibodyhMAD-2 antibodyhSMAD2 antibody

  JV18 1 antibodyJV18 antibodyJV18 antibodyJV18-1 antibodyJV181 antibodyMAD antibodyMAD antibodyMAD homolog 2 antibodyMAD Related Protein 2 antibodyMad-related protein 2 antibodyMADH2 antibodyMADR2 antibodyMGC22139 antibodyMGC34440 antibodyMothers Against Decapentaplegic Homolog 2 antibodyMothers Against Decapentaplegic Homolog 2 antibodymothers against DPP homolog 2 antibodyOTTHUMP00000163489 antibodySma and Mad related protein 2 antibodySMAD 2 antibodySMAD antibodySMAD family member 2 antibodySMAD, mothers against DPP homolog 2 antibodySMAD2 antibodySMAD2_HUMAN antibody

  see all

Anti-Smad2 antibody [EP567Y] images

• 

  Western blot - Smad2 antibody [EP567Y] (ab33875)
  Anti-Smad2 antibody [EP567Y] (ab33875) at 1/1000 dilution + Jurkat cell lysate
  
  Predicted band size : 58 kDa
  Observed band size : 58 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - Smad2 antibody [EP567Y] (ab33875)
  ab33875, at a 1/50 dilution,staining human adenocarcinoma of uterus by Immunohistochemistry, Paraffin embedded tissue.
• 

  Western blot - Smad2 antibody [EP567Y] (ab33875)
  All lanes : Anti-Smad2 antibody [EP567Y] (ab33875) at 1/3000 dilution
  
  Lane 1 : HGL-5 cells - no additives
  Lane 2 : Cells + activin A
  Lane 3 : Cells + cyclophilin siRNA Control
  Lane 4 : Cells + activin A + cyclophilin
  Lane 5 : non-targeting, negative control for siRNA
  Lane 6 : Cells + activin A, negative control for siRNA
  Lane 7 : Cells + siRNA Smartpool
  Lane 8 : Cells + activin A + siRNA Smartpool
  Lane 9 : Jurkat cells
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/50000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 58 kDa
  Observed band size : 58 kDa
  

  This image is courtesy of an Abreview submitted by Mrs Birte Jablonski

  See Abreview

• 

  Flow Cytometry - Smad2 antibody [EP567Y] (ab33875)
  Overlay histogram showing PC3 cells stained with ab33875 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33875, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.