Overview

  • Product nameAnti-Smad2 antibody [EP784Y]See all Smad2 primary antibodies ...
  • Description
    Rabbit monoclonal [EP784Y] to Smad2
  • SpecificityThis antibody is specific for MH 1 domain of Smad2.
  • Tested applicationsICC, IHC-P, IP, WB, Flow Cyt more details
  • Species reactivity
    Reacts with: Rat, Human

    Does not react with

    Mouse
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Jurkat cell lysate and human prostate carcinoma tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab40855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
IP 1/50.
WB 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 58 kDa).
Flow Cyt 1/100.

Target

  • FunctionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
  • Tissue specificityExpressed at high levels in skeletal muscle, heart and placenta.
  • Sequence similaritiesBelongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications
    Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
    In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
    Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
  • Target information above from: UniProt accession Q15796 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • hMAD 2 antibody
    • hMAD-2 antibody
    • hSMAD2 antibody
    • JV18 1 antibody
    • JV18 antibody
    • JV18 antibody
    • JV18-1 antibody
    • JV181 antibody
    • MAD antibody
    • MAD antibody
    • MAD homolog 2 antibody
    • MAD Related Protein 2 antibody
    • Mad-related protein 2 antibody
    • MADH2 antibody
    • MADR2 antibody
    • MGC22139 antibody
    • MGC34440 antibody
    • Mother against DPP homolog 2 antibody
    • Mothers Against Decapentaplegic Homolog 2 antibody
    • Mothers Against Decapentaplegic Homolog 2 antibody
    • mothers against DPP homolog 2 antibody
    • OTTHUMP00000163489 antibody
    • Sma and Mad related protein 2 antibody
    • SMAD 2 antibody
    • SMAD antibody
    • SMAD family member 2 antibody
    • SMAD, mothers against DPP homolog 2 antibody
    • SMAD2 antibody
    • SMAD2_HUMAN antibody
    see all

Anti-Smad2 antibody [EP784Y] images

  • Overlay histogram showing PC3 cells stained with ab40855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40855, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-Smad2 antibody [EP784Y] (ab40855) at 1/500000 dilution + Jurkat cell lysate

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ab40855 at a 1:100 dilution staining Smad2 in human prostate carcinoma tissue.ab40855 at a 1:100 dilution staining Smad2 in human prostate carcinoma tissue.

References for Anti-Smad2 antibody [EP784Y] (ab40855)

This product has been referenced in:
  • Harazono Y  et al. miR-655 Is an EMT-suppressive MicroRNA targeting ZEB1 and TGFBR2. PLoS One 8:e62757 (2013). WB ; Human . Read more (PubMed: 23690952) »
  • Sundqvist A  et al. Specific interactions between Smad proteins and AP-1 components determine TGFß-induced breast cancer cell invasion. Oncogene : (2012). Read more (PubMed: 22926518) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Penile Tissue)
Loading amount 60 µg
Specification Penile Tissue
Gel Running Conditions Reduced Denaturing (4-20% mini gel)
Blocking step SuperBlock Blocking Buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Jul 26 2012

Application Western blot
Sample Human Cell lysate - whole cell (Epithelial, neutrophil, monocyte)
Loading amount 500000 cells
Specification Epithelial, neutrophil, monocyte
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2009

Application Western blot
Sample Rat Tissue lysate - whole (Penile Tissue)
Loading amount 80 µg
Specification Penile Tissue
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step Starting Block T20 (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Jul 29 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"