Anti-Smad2 antibody (ab11784)

Overview

• Product nameAnti-Smad2 antibodySee all Smad2 primary antibodies ...
• Description
  Goat polyclonal to Smad2
• SpecificityThis antibody recognises the human MADH2 protein, also known as SMAD2. This protein is a transcriptional modulator activated by TGF beta and may act as a tumour suppressor in colorectal carcinoma. It is found at high levels in skeletal muscle, heart and placenta.
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Chicken, Xenopus laevis, Goldfish
• Immunogen

  Synthetic peptide: SEIWGLSTPNTID (Goat) corresponding to amino acids 82-94 derived from MADH2 protein sequence.

  Run BLAST with Run BLAST with
• Positive controlHeLa cell lysates. This antibody gave a positive result in IF in the following Methanol fixed cell line: MCF-7.
• General notesStorage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Whole serum, Tris buffered saline, pH 7.3
• Concentration information loading...
• PurityProtein A purified
• Purification notesPurification prepared by affinity chromatography.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Cancer
  • Growth factors
  • TGF

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • SMADs

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • SMADs

Applications

Target

• FunctionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
• Tissue specificityExpressed at high levels in skeletal muscle, heart and placenta.
• Sequence similaritiesBelongs to the dwarfin/SMAD family.
  Contains 1 MH1 (MAD homology 1) domain.
  Contains 1 MH2 (MAD homology 2) domain.
• Post-translational
  modificationsPhosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
  In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
  Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
• Cellular localizationCytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.

Target information above from: UniProt accession Q15796 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4087 Human
  • Omim: 601366 Human
  • SwissProt: Q15796 Human
  • Unigene: 12253 Human
  • Unigene: 705764 Human

• Alternative names
  hMAD 2 antibodyhMAD-2 antibodyhSMAD2 antibody

  JV18 1 antibodyJV18 antibodyJV18-1 antibodyJV181 antibodyMAD antibodyMAD homolog 2 antibodyMAD Related Protein 2 antibodyMad-related protein 2 antibodyMADH2 antibodyMADR2 antibodyMGC22139 antibodyMGC34440 antibodyMothers Against Decapentaplegic Homolog 2 antibodymothers against DPP homolog 2 antibodyOTTHUMP00000163489 antibodySma and Mad related protein 2 antibodySMAD 2 antibodySMAD antibodySMAD family member 2 antibodySMAD, mothers against DPP homolog 2 antibodySMAD2 antibodySMAD2_HUMAN antibody

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Anti-Smad2 antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (ab11784)

  ICC/IF image of ab11784 stained MCF-7 cells. The cells were 100% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11784 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.