Overview
- Product nameAnti-Smad2 antibodySee all Smad2 primary antibodies ...
- DescriptionRabbit polyclonal to Smad2
- Tested applicationsIHC-Fr, WB, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Chimpanzee
Predicted to work with: a wide range of other species - Immunogen
Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 protein.
- Positive controlRat or mouse muscle lysate
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS -
Concentration information loading... - PurityProtein G purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab47083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-Fr | IHC-Fr: 1/30. PubMed: 19556713 |
| WB | WB: Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 52 kDa). |
| IHC-P | IHC-P: Use at an assay dependent dilution. PubMed: 20962090 |
Target
- FunctionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
- Tissue specificityExpressed at high levels in skeletal muscle, heart and placenta.
- Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. - Post-translational
modificationsPhosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta. - Cellular localizationCytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
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Database links
- Entrez Gene: 516010 Cow
- Entrez Gene: 4087 Human
- Entrez Gene: 17126 Mouse
- Entrez Gene: 29357 Rat
- Omim: 601366 Human
- SwissProt: Q1W668 Cow
- SwissProt: Q15796 Human
- SwissProt: Q62432 Mouse
- SwissProt: O70436 Rat
- Unigene: 12253 Human
- Unigene: 705764 Human
- Unigene: 391091 Mouse
- Unigene: 2755 Rat
see all
Target information above from: UniProt accession
Q15796
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- hMAD 2 antibodyhMAD-2 antibodyhSMAD2 antibody
- JV18 1 antibodyJV18 antibodyJV18-1 antibodyJV181 antibodyMAD antibodyMAD homolog 2 antibodyMAD Related Protein 2 antibodyMad-related protein 2 antibodyMADH2 antibodyMADR2 antibodyMGC22139 antibodyMGC34440 antibodyMothers Against Decapentaplegic Homolog 2 antibodymothers against DPP homolog 2 antibodyOTTHUMP00000163489 antibodySma and Mad related protein 2 antibodySMAD 2 antibodySMAD antibodySMAD family member 2 antibodySMAD, mothers against DPP homolog 2 antibodySMAD2 antibodySMAD2_HUMAN antibody
see all
Anti-Smad2 antibody images
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Western blot - Smad2 antibody (ab47083)All lanes : Anti-Smad2 antibody (ab47083) at 3 µg/ml
Lane 1 : rat muscle lysate
Lane 2 : rat muscle lysate in the presence of immunizing peptide.
Predicted band size : 52 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)
Additional bands at : 21 kDa,70 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Smad2 antibody (ab47083)
This product has been referenced in:
- Xu S et al. Marek's disease virus type 1 microRNA miR-M3 suppresses cisplatin-induced apoptosis by targeting Smad2 of the transforming growth factor beta signal pathway. J Virol 85:276-85 (2011). WB, IHC-P ; Chicken . Read more (PubMed: 20962090) »
- Nachtigal P et al. Atorvastatin Increases Endoglin, SMAD2, Phosphorylated SMAD2/3 and eNOS Expression in ApoE/LDLR Double Knockout Mice. J Atheroscler Thromb 16:265-74 (2009). WB, IHC-Fr ; Mouse . Read more (PubMed: 19556713) »
