Anti-Smad2 (phospho S250) antibody (ab79179)

Overview

• Product nameAnti-Smad2 (phospho S250) antibodySee all Smad2 primary antibodies ...
• Description
  Rabbit polyclonal to Smad2 (phospho S250)
• Specificityab79179 detects endogenous levels of Smad2 only when phosphorylated at serine 250.
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of serine 250 (E-L-S^P-P-T).

• Positive control293 cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Cytoplasmic

  • Cancer
  • Growth factors
  • TGF

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • SMADs

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • SMADs

Applications

Target

• FunctionReceptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
• Tissue specificityExpressed at high levels in skeletal muscle, heart and placenta.
• Sequence similaritiesBelongs to the dwarfin/SMAD family.
  Contains 1 MH1 (MAD homology 1) domain.
  Contains 1 MH2 (MAD homology 2) domain.
• Post-translational
  modificationsPhosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
  In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
  Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
• Cellular localizationCytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.

Target information above from: UniProt accession Q15796 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4087 Human
  • Entrez Gene: 17126 Mouse
  • Entrez Gene: 29357 Rat
  • Omim: 601366 Human
  • SwissProt: Q15796 Human
  • SwissProt: Q62432 Mouse
  • SwissProt: O70436 Rat
  • Unigene: 12253 Human

  • Unigene: 705764 Human
  • Unigene: 391091 Mouse
  • Unigene: 2755 Rat

  see all

• Alternative names
  hMAD 2 antibodyhMAD-2 antibodyhSMAD2 antibody

  JV18 1 antibodyJV18 antibodyJV18-1 antibodyJV181 antibodyMAD antibodyMAD homolog 2 antibodyMAD Related Protein 2 antibodyMad-related protein 2 antibodyMADH2 antibodyMADR2 antibodyMGC22139 antibodyMGC34440 antibodyMothers Against Decapentaplegic Homolog 2 antibodymothers against DPP homolog 2 antibodyOTTHUMP00000163489 antibodySma and Mad related protein 2 antibodySMAD 2 antibodySMAD antibodySMAD family member 2 antibodySMAD, mothers against DPP homolog 2 antibodySMAD2 antibodySMAD2_HUMAN antibody

  see all

Anti-Smad2 (phospho S250) antibody images

• 

  Western blot - Smad2 (phospho S250) antibody (ab79179)
  All lanes : Anti-Smad2 (phospho S250) antibody (ab79179)
  
  Lane 1 : 293 cells treated with PMA (125 ng/ml, 30 minutes)
  Lane 2 : 293 cells treated with PMA (125 ng/ml, 30 minutes) with Phospho peptide
  
  
  Predicted band size : 52 kDa
  Observed band size : 60 kDa (why is the actual band size different from the predicted?)