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Recombinant full length Smad3 protein (Human) purified from E.coli (His-Smad3)
Our Abpromise guarantee covers the use of ab75512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|Flow Cyt||1/100. Ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP568Y] to Smad3 (ab40854). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit monoclonal [EP568Y] to Smad3 (ab40854) as Detection.|
|WB||1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 48 kDa).|
|ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"