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Synthetic peptide corresponding to the internal region of Human Smad3.
Our Abpromise guarantee covers the use of ab40854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).Can be blocked with Smad3 peptide (ab173094).|
|ICC||1/100 - 1/250.|
|IHC-P||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [AF9F7] to Smad3 (ab75512). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AF9F7] to Smad3 (ab75512) as Capture.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"