Smad3 antibody [EP568Y] (ab40854)

Product Name 

Smad3 antibody [EP568Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP568Y] to Smad3

Immunogen 

Synthetic peptide corresponding to the internal region of Human Smad3.

Reacts with 

Hu, Rat

Does not react with 
(species key)

Ms

Specificity 

This antibody is specific for Smad3.

Tested applications 
(see key)

ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cell lysate and human prostate carcinoma tissue.

Cellular localization 

In the cytoplasm in the absence of ligand; migration to the nucleus when complexed with SMAD4.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Signal transduction >> Nuclear signaling >> SMAD family
Stem Cells >> Signaling Pathways >> TGF beta >> Cytoplasmic
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> SMADs
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> SMADs

Relevance 

Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP568Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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