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Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Smad3.
(Peptide available as ab95046.)
Our Abpromise guarantee covers the use of ab84177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human Smad3 peptide (ab95046).|
|IHC-P||Use a concentration of 1 µg/ml.|
ab84177 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"