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All lanes : Anti-Smad3 (phospho S204) antibody (ab63402) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells,
treated with Serum (20%, 15mins)
Lane 2 : Extracts from NIH/3T3 cells,
treated with Serum (20%, 15mins) plus phospho peptide
Predicted band size : 48 kDa
Observed band size : 48 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide is 5-10 ug.
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