Useful resources:
- Blocking with immunizing peptide protocol (BL)
- Buffers and stock solutions
- Fluorophore table
- Troubleshooting tips
Western blotting (WB) protocols:
- Western blotting (WB) a beginner's guide
- Stripping for reprobing
- Western blotting of phospho-proteins protocol & tips
- Nuclear fractionation protocol
- Histone extraction protocol
- Histone western blotting protocol
- Mitochondrial purification protocol
- Soluble (S100) mitochondrial fractionation protocol
- Determining if the antibody binds only phosphorylated proteins
- Having problems?
Download our detailed troubleshooting tips for WB
Immunohistochemistry (IHC) / Immunocytochemistry (ICC) protocols:
- IHC-Fr staining protocol
- IHC-P staining protocol
- ICC (IF) protocol
- Double immunofluorescence - sequential protocol
- Double immunofluorescence - simultaneous protocol
- Determining if antibody binds only phosphorylated protein
- Fixation and permeabilization for IHC and ICC
- GMA embedding for immunohistochemistry
- Mouse on mouse staining procedure
- Perfusion fixation protocol
- Troubleshooting tips
Chromatin Immunoprecipitation (ChIP) protocols:
Dot blot protocols:
ELISA protocols:
- Direct ELISA protocol using primary antibody
- Direct ELISA protocol using fluorescent substrate
- Indirect ELISA protocol using primary and secondary antibodies
- Sandwich ELISA protocol using a pair of primary antibodies
ELISPOT protocols:
Flow cytometry / FACS protocols:
- Flow cytometry - a guide / introduction
- Direct flow cytometry (FACS) protocol
- Indirect flow cytometry (FACS) protocol
- Flow cytometry intracellular staining protocol
- Flow cytometry recommended controls
- Red blood cell lysis protocol
- Having problems?
Download our detailed flow cytometry troubleshooting tips!
![Western blot - Smad3 antibody [EP823Y] (ab52903)](/ps/datasheet/Images/52/ab52903/ab52903_wb.jpg)
![Immunocytochemistry/ Immunofluorescence - Smad3 antibody [EP823Y] (ab52903)](/ps/datasheet/Images/52/ab52903/ab52903_ICC.jpg)
![Immunohistochemistry - Smad3 (phospho S423 + S425) antibody [EP823Y] (ab52903)](/ps/datasheet/Images/52/ab52903/ab52903_IHC.jpg)
![Western blot - Smad3 (phospho S423 + S425) antibody [EP823Y] (ab52903)](/ps/datasheet/images/52/ab52903/Smad3-Primary-antibodies-ab52903-5.jpg)
![Immunocytochemistry/ Immunofluorescence - Smad3 (phospho S423 + S425) antibody [EP823Y] (ab52903)](/ps/datasheet/images/52/ab52903/Smad3-Primary-antibodies-ab52903-6.jpg)
![Smad3 (phospho S423 + S425) antibody [EP823Y] for WB in Human (52903)](/ps/datasheet/images/52/ab52903/Smad3-Primary-antibodies-ab52903-1.png)
![Smad3 (phospho S423 + S425) antibody [EP823Y] for WB in Human (52903)](/ps/datasheet/images/52/ab52903/Smad3-Primary-antibodies-ab52903-4.JPG)
![Smad3 (phospho S423 + S425) antibody [EP823Y] for ELISA in Human (52903)](/ps/datasheet/images/52/ab52903/Smad3-Primary-antibodies-ab52903-8.jpg)
![Smad3 (phospho S423 + S425) antibody [EP823Y] for ICC/IF in Human (52903)](/ps/datasheet/images/52/ab52903/Smad3-Primary-antibodies-ab52903-3.png)
![Smad3 (phospho S423 + S425) antibody [EP823Y] for Western blot in Human (52903)](/ps/datasheet/images/52/ab52903/Smad3-Primary-antibodies-ab52903-11.jpg)