Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> SMADs
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ab52903 has been referenced in 6 publications.
Publishing research using ab52903? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-Smad3 (phospho S423 + S425) antibody [EP823Y] (ab52903) at 1/2000 dilution
Lane 1 : (A) HL-60 cell lysates at 10µg untreated
Lane 2 : (B) HL-60 cell lysates at 10µg treated with TGF.
Predicted band size : 48 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescent staining of Smad3 in HeLa cells using ab52903 at 1/100 dilution.
Immunohistochemical analysis of Smad3 in paraffin embedded human liver carcinoma tissue using ab52903 at 1/100 dilution.
All lanes : Anti-Smad3 (phospho S423 + S425) antibody [EP823Y] (ab52903) at 1/1000 dilution
Lane 1 : Lysate prepared from untreated human A549 cells
Lane 2 : Lysate prepared from untreated human A549 cells for 30min
Lane 3 : Lysate prepared from TGF-ß1 cells at 10ng/ml for 30min
Lane 4 : Lysate prepared from TNF-a cells at 20ng/ml for 30min
Lane 5 : Lysate prepared from TGF-ß1 and TNF-a cells at above doses for 30min
Lane 6 : Blank DMEM media
Lysates/proteins at 20 µg per lane.
Secondary
Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284)
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 48 kDa
Observed band size : 48 kDa
Exposure time : 1 hour
This image is a courtesy of Aaron Gardner
ab52903 staining Smad3 (phospho S423 + S425) in human TII Pneumocyte A549c ells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton x100 before blocking with 3% BSA for 1 hour at RT. Samples were incubated with primary antibody (1/200: in 3% BSA in 1x PBST) for 24 hours at 4°C. A TRITC-conjugated goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.
This image is a courtesy of Aaron Gardner
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