Overview

  • Product name
  • Description
    Rabbit polyclonal to Smek1
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 770 and the C-terminus (residue 820) of Human Smek1 (NP_115949.3)

  • Positive control
    • Whole cell lysate from HeLa, 293T and NIH3T3 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
  • Sequence similarities
    Belongs to the SMEK family.
    Contains 1 WH1 domain.
  • Cellular localization
    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. In interphase localized in the cytoplasm and in the nucleus (with higher levels). During metaphase located in pericentriolar regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLFL1 antibody
    • KIAA2010 antibody
    • MEK1 SUPPRESSOR 1 antibody
    • MSTP033 antibody
    • P4R3A_HUMAN antibody
    • PP4R3 ALPHA antibody
    • PP4R3 antibody
    • PP4R3A antibody
    • PPP4R3A antibody
    • Protein phosphatase 4 regulatory subunit 3A antibody
    • PROTEIN PHOSPHATASE 4, REGULATORY SUBUNIT 3, ALPHA antibody
    • Serine/threonine-protein phosphatase 4 regulatory subunit 3A antibody
    • SMEK homolog 1 antibody
    • SMEK homolog 1, suppressor of mek1 (Dictyostelium) antibody
    • smek1 antibody
    • Smk 1 antibody
    • SMK1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and breast carcinoma (right) tissues labelling Smek1 with ab70635 at 1/200 (1µg/ml) and 1/80 (2.5µg/ml). Detection: DAB and DyLight® 594-conjugated goat anti-rabbit IgG (1/100).
  • All lanes : Anti-Smek1 antibody (ab70635) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 95 kDa
    Observed band size : 95 kDa


    Exposure time : 10 seconds
  • Detection of Smek1 by Western Blot of Immunprecipitate. ab70635 at 1µg/ml staining Smek1 in HeLa whole cell lysate immunoprecipitated using ab70635 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 0.5 seconds.

References

ab70635 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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