Overview

  • Product name
  • Description
    Rabbit polyclonal to Smg1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Orangutan, Platypus
  • Immunogen

    Synthetic peptide within Human Smg1 aa 1250-1300. The exact sequence is proprietary.
    Database link: NP_055907.3

  • Positive control
    • HeLa cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 410 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
  • Tissue specificity
    Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
  • Sequence similarities
    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 1 HEAT repeat.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 61E3.4 antibody
    • ATX antibody
    • hSMG-1 antibody
    • hSMG1 antibody
    • KIAA0421 antibody
    • Lambda interacting protein antibody
    • Lambda-interacting protein antibody
    • Lambda/iota protein kinase C interacting protein antibody
    • Lambda/iota protein kinase C-interacting protein antibody
    • LIP antibody
    • Phosphatidylinositol 3 kinase related kinase antibody
    • Phosphatidylinositol 3 kinase related protein kinase antibody
    • PI 3 kinase related kinase SMG 1 antibody
    • Serine/threonine protein kinase SMG1 antibody
    • Serine/threonine-protein kinase SMG1 antibody
    • SMG-1 antibody
    • smg1 antibody
    • SMG1_HUMAN antibody
    see all

Images

  • All lanes : Anti-Smg1 antibody (ab80600) at 1 µg/ml

    Lane 1 : Whole HeLa cell lysate at 50 µg
    Lane 2 : Whole HeLa cell lysate at 15 µg

    Developed using the ECL technique

    Predicted band size : 410 kDa
    Observed band size : 410 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human Smg1 by Immunoprecipitation. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab80600 used for IP at 3 mcg/mg lysate. Subsequent Wb detection with ab80600 used at 1 mcg/ml. Chemiluminescence with exposure times of 3 mins

References

This product has been referenced in:
  • Henderson-Smith A  et al. SMG1 identified as a regulator of Parkinson's disease-associated alpha-synuclein through siRNA screening. PLoS One 8:e77711 (2013). WB . Read more (PubMed: 24204929) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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