Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
Lambda/iota protein kinase C interacting protein antibody
Lambda/iota protein kinase C-interacting protein antibody
Phosphatidylinositol 3 kinase related kinase antibody
Phosphatidylinositol 3 kinase related protein kinase antibody
PI 3 kinase related kinase SMG 1 antibody
Serine/threonine protein kinase SMG1 antibody
Serine/threonine-protein kinase SMG1 antibody
Western blot - Smg1 antibody (ab80600)
All lanes : Anti-Smg1 antibody (ab80600) at 1 µg/ml
Lane 1 : Whole HeLa cell lysate at 50 µg Lane 2 : Whole HeLa cell lysate at 15 µg
Developed using the ECL technique
Predicted band size : 410 kDa Observed band size : 410 kDa Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Smg1 antibody (ab80600)
Detection of Human Smg1 by Immunoprecipitation. Sample: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab80600 used for IP at 3 mcg/mg lysate. Subsequent Wb detection with ab80600 used at 1 mcg/ml. Chemiluminescence with exposure times of 3 mins