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Smoothelin protein (Tagged-His Tag) (ab92256)

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Overview

  • Product nameSmoothelin protein (Tagged-His Tag)See all Smoothelin proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 707-915 of Human Smoothelin with N terminal His tag; 209 amino acids, 26kDa (23.4kDa predicted from sequence). UniProt ID = P53814.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 126 µl aqua dest.
  • Storage instructionsStore at 4°C. Upon reconstitution store at -80ºC.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesKTFSSSSSSKKMGSIFDREDQASPRAGSLAALEKRQAEKK KELMKAQSLPKTSASQARKA MIEKLEKEGAAGSPGGPR AAVQRSTSFGVPNANSIKQMLLDWCRAKTRGYEHVDIQNF SS SWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEV AFSSAETHADCPQLLDTEDMVRLR EPDWKCVYTYIQEF YRCLVQKGLVKTKKS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92256 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        FLJ35168FLJ35365FLJ35620
        FLJ38597smoothelinSMSMOSMTNSMTN_HUMAN
      see all
  • FunctionStructural protein of the cytoskeleton.
  • Tissue specificitySmooth muscle; contractile or vascular (for the long form).
  • Sequence similaritiesBelongs to the smoothelin family.
    Contains 1 CH (calponin-homology) domain.
  • Cellular localizationCytoplasm > cytoskeleton. Exhibits a filamentous organization.

    • Target information above from: UniProt accession P53814 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Smoothelin protein (Tagged-His Tag) images

    • SDS-PAGE - Smoothelin protein (Tagged-His Tag) (ab92256)
      SDS-PAGE - Smoothelin protein (Tagged-His Tag) (ab92256)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Smoothelin protein (Tagged-His Tag) (ab92256)

    ab92256 has not yet been referenced specifically in any publications.

    Publishing research using ab92256 ? Please let us know so that we can cite the reference in this datasheet

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