Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314)
Key features and details
- Rabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1
- Suitable for: IHC-P, WB, ICC, ICC/IF, IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody
See all Sodium/Hydrogen Exchanger 1/NHE-1 primary antibodies -
Description
Rabbit polyclonal to Sodium/Hydrogen Exchanger 1/NHE-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC, ICC/IF, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Sodium/Hydrogen Exchanger 1/NHE-1 (internal sequence). The immunogen is located within amino acids 490 - 540 of Sodium/Hydrogen Exchanger 1/NHE-1.
Database link: P19634 -
Positive control
- WB: MOLT4 cell lysate. IHC-P: Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 2.5 µg/ml.
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
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ICC |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 20817726
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 20712402
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Notes |
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IHC-P
Use a concentration of 2.5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa). |
ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 20817726 |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20712402 |
Target
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Function
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. -
Tissue specificity
Kidney and intestine. -
Sequence similarities
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6548 Human
- Entrez Gene: 20544 Mouse
- Entrez Gene: 24782 Rat
- Omim: 107310 Human
- SwissProt: P19634 Human
- SwissProt: Q61165 Mouse
- SwissProt: P26431 Rat
- Unigene: 469116 Human
see all -
Alternative names
- amiloride-sensitive antibody
- APNH antibody
- APNH1 antibody
see all
Images
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Lane 1 : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 1 µg/ml
Lane 2 : Anti-Sodium/Hydrogen Exchanger 1/NHE-1 antibody (ab67314) at 2 µg/ml
All lanes : MOLT4 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 91 kDa
Observed band size: 91 kDa
Additional bands at: 48 kDa (possible isoform) -
Immunohistochemical staining of Sodium / Hydrogen Exchanger 1 in human brain tissue using ab67314 at 2.5 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab67314 has been referenced in 14 publications.
- Deguchi H et al. Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy. Sci Rep 12:6263 (2022). PubMed: 35428816
- Lee HP et al. The nuclear piston activates mechanosensitive ion channels to generate cell migration paths in confining microenvironments. Sci Adv 7:N/A (2021). PubMed: 33523987
- Wang Y et al. Correlation between electrical characteristics and biomarkers in breast cancer cells. Sci Rep 11:14294 (2021). PubMed: 34253828
- Riaz S et al. Anti-hypertrophic effect of Na+/H+ exchanger-1 inhibition is mediated by reduced cathepsin B. Eur J Pharmacol 888:173420 (2020). PubMed: 32781168
- Yang D et al. Normobaric oxygen inhibits AQP4 and NHE1 expression in experimental focal ischemic stroke. Int J Mol Med 43:1193-1202 (2019). PubMed: 30592266