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Anti-Soluble Liver/Pancreas Antigen antibody (ab56033) at 1 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 49 kDa
Observed band size : 54 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
ab56033, at 8 µg/ml, staining Human Soluble Liver/Pancreas Antigen in kidney by immunohistochemistry. Paraffin embedded tissue. Arrows indicate positively stained epithelial cells of the renal tubule. X400 magnification.
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