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Anti-Soluble TNF Receptor II antibody [22221.311] (ab10503)

Submit an Abreview Q&A (1)Specific References (1)

Overview

  • Product nameAnti-Soluble TNF Receptor II antibody [22221.311]See all Soluble TNF Receptor II primary antibodies ...
  • Description
    Mouse monoclonal [22221.311] to Soluble TNF Receptor II
  • SpecificityBy immunoblotting and ELISA, the antibody shows no cross reactivity with recombinant human sTNF-RI.
  • Tested applicationsELISA, WB, Neutralising more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human sTNF-RII, expressed in E. coli.

  • General notesEndotoxin level is < 10 ng per mg antibody as determined by the LAL method.

Properties

Applications

Our Abpromise guarantee covers the use of ab10503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
WB
Neutralising
  • Application notesELISA: Use at a concentration of 0.5 - 1 µg/ml, this concentration is determined to detect recombinant human sTNF RII to a limit of 1.25 ng/well.
    Neut: Use at a concentration of 0.5 - 1 µg/ml, this concentration will block 50% of the bioactivity due to 0.3 µg/ml recombinant human sTNF RII, in the presence of 0.25 ng/ml TNF-alpha in a crystal violet cytolytic assay using mouse L929 cells.
    WB: Use at a concentration of 1 - 2 µg/ml, this concentration is determined using recombinant human sTNF RII at 50 ng/lane under non-reducing conditions.Predicted molecular weight: 75 kDa.

    Anti-Human sTNF RII is tested for its ability to neutralize the biological effect of sTNF-RII but not sTNF RI in a cytolytic assay using mouse L929 cells, in the presence of TNF-alpha. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of the soluble receptor activity on a responsive cell line, when the soluble receptor is present at a concentration just high enough to elicit a maximum response.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
    • Sequence similaritiesContains 4 TNFR-Cys repeats.
    • Post-translational
      modifications      Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
      A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
    • Cellular localizationSecreted and Cell membrane.

      • Target information above from: UniProt accession P20333 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CD120b antibodyEtanercept antibodyp75 antibody
          p75 TNF receptor antibodyp75TNFR antibodyp80 TNF alpha receptor antibodyp80 TNF-alpha receptor antibodySoluble TNFR1B variant 1 antibodyTBP-2 antibodyTBPII antibodyTNF R II antibodyTNF R2 antibodyTNF R2 antibodyTNF R75 antibodyTNF-R2 antibodyTNF-RII antibodyTNFBR antibodyTNFR-II antibodyTNFR1B antibodyTNFR2 antibodyTNFR80 antibodyTnfrsf1b antibodyTNR1B_HUMAN antibodyTumor necrosis factor beta receptor antibodyTumor necrosis factor binding protein 2 antibodyTumor necrosis factor receptor 2 antibodyTumor necrosis factor receptor superfamily member 1B antibodyTumor necrosis factor receptor superfamily, member 1B antibodyTumor necrosis factor receptor type II antibodyTumor necrosis factor-binding protein 2 antibody
        see all

      References for Anti-Soluble TNF Receptor II antibody [22221.311] (ab10503)

      This product has been referenced in:
      • Bhattacharyya S  et al. Tumor Necrosis Factor {alpha}-induced Inflammation Is Increased but Apoptosis Is Inhibited by Common Food Additive Carrageenan. J Biol Chem 285:39511-22 (2010). Read more (PubMed: 20937806) »

      See 1 Publication for this product

      Publishing research using ab10503 ? Please let us know so that we can cite the reference in this datasheet

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      I just want to know more information about your antibody for human soluble TNFRII. ab10503, ab10502. Can you give me the amino acids these two antibodies recoganize? Your recommended molecular weight for sTNFRII is 75Kd, is it right? As you know, the mol...

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      Thank you for your enquiry. For both products, the immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the tr...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"