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Anti-Soluble TNF Receptor II antibody (ab10502)

Abreviews (1)Q&A (1)

Overview

  • Product nameAnti-Soluble TNF Receptor II antibodySee all Soluble TNF Receptor II primary antibodies ...
  • Description
    Goat polyclonal to Soluble TNF Receptor II
  • SpecificityBy ELISA, the antibody shows no cross-reactivity with recombinant human sTNF-RI. In addition, the antibody shows no cross-reactivity with other cytokines tested: rhTGF-beta1, pTGF-beta1.2, pTGF-beta2, rcTGF-beta3, raTGF-beta5, rhLatent TGF-beta1, rhTGF-alpha, rhEGF, rhPDGF-AA, rhPDGF-BB, rhPDGF-AB, rhFGF acidic, rhFGF basic, rhFGF-4, rhIL-1a, rmIL-1a, rhIL-1ra, rhIL-1b, rmIL-1b, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, rhIL-11, rhGM-CSF, rhM-CSF, rhLIF, rhIL-6 sR, rhMIP-1alpha, rmMIP-1alpha, rhMIP-1beta, rmMIP-1beta, rhTNF-alpha, rmTNF-alpha, rhTNF-beta, rhRANTES, rhANG, rhOSM, rhSCF, rmSCF, rhSLPI, rhCNTF, rhIFN-gamma, rhIGF-I, rhIGF-II, rhGRO, rhEpo, rhMCP-1.
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human TNF soluble receptor II (TNF sRII), expressed in E. coli. This recombinant protein represents the non-glycosylated,N-terminal methionyl form of the naturally occuring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the transmembrane domain.

  • General notesEndotoxin level is < 10 ng per mg antibody as determined by the LAL method.

Properties

Applications

Our Abpromise guarantee covers the use of ab10502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
WB
  • Application notesELISA: Use at a concentration of 0.5 - 1 µg/ml, this is determined to detect recombinant human sTNF RII to a limit of 78 pg/well.
    WB: Use at a concentration of 1 - 2 µg/ml, this is determined using recombinant human sTNF RII at 5 ng/lane under non-reducing conditions. Predicted molecular weight: 75 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
    • Sequence similaritiesContains 4 TNFR-Cys repeats.
    • Post-translational
      modifications      Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
      A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
    • Cellular localizationSecreted and Cell membrane.

      • Target information above from: UniProt accession P20333 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CD120b antibodyEtanercept antibodyp75 antibody
          p75 TNF receptor antibodyp75TNFR antibodyp80 TNF alpha receptor antibodyp80 TNF-alpha receptor antibodySoluble TNFR1B variant 1 antibodyTBP-2 antibodyTBPII antibodyTNF R II antibodyTNF R2 antibodyTNF R75 antibodyTNF-R2 antibodyTNF-RII antibodyTNFBR antibodyTNFR-II antibodyTNFR1B antibodyTNFR2 antibodyTNFR80 antibodyTNFRSF1B antibodyTNR1B_HUMAN antibodyTumor necrosis factor beta receptor antibodyTumor necrosis factor binding protein 2 antibodyTumor necrosis factor receptor 2 antibodyTumor necrosis factor receptor superfamily member 1B antibodyTumor necrosis factor receptor superfamily, member 1B antibodyTumor necrosis factor receptor type II antibodyTumor necrosis factor-binding protein 2 antibody
        see all

      References for Anti-Soluble TNF Receptor II antibody (ab10502)

      ab10502 has not yet been referenced specifically in any publications.

      Publishing research using ab10502 ? Please let us know so that we can cite the reference in this datasheet

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      Western blot abreview for Anti-Soluble TNF Receptor II antibody

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      Abreviews
      Application Western blot
      Sample Human Cell lysate - whole cell (Hepatoma cells)
      Loading amount 10 µg
      Specification Hepatoma cells
      Treatment 2 ug TNFR2 for 24h
      Gel Running Conditions Reduced Denaturing (12%)
      Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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      Verified customer

      Submitted Jul 09 2010

      I just want to know more information about your antibody for human soluble TNFRII. ab10503, ab10502. Can you give me the amino acids these two antibodies recoganize? Your recommended molecular weight for sTNFRII is 75Kd, is it right? As you know, the mol...

      Read More

      Thank you for your enquiry. For both products, the immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the tr...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"