Anti-Soluble TNF Receptor II antibody (ab10502)

Overview

  • Product nameAnti-Soluble TNF Receptor II antibodySee all Soluble TNF Receptor II primary antibodies ...
  • Description
    Goat polyclonal to Soluble TNF Receptor II
  • SpecificityBy ELISA, the antibody shows no cross-reactivity with recombinant human sTNF-RI. In addition, the antibody shows no cross-reactivity with other cytokines tested: rhTGF-beta1, pTGF-beta1.2, pTGF-beta2, rcTGF-beta3, raTGF-beta5, rhLatent TGF-beta1, rhTGF-alpha, rhEGF, rhPDGF-AA, rhPDGF-BB, rhPDGF-AB, rhFGF acidic, rhFGF basic, rhFGF-4, rhIL-1a, rmIL-1a, rhIL-1ra, rhIL-1b, rmIL-1b, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, rhIL-11, rhGM-CSF, rhM-CSF, rhLIF, rhIL-6 sR, rhMIP-1alpha, rmMIP-1alpha, rhMIP-1beta, rmMIP-1beta, rhTNF-alpha, rmTNF-alpha, rhTNF-beta, rhRANTES, rhANG, rhOSM, rhSCF, rmSCF, rhSLPI, rhCNTF, rhIFN-gamma, rhIGF-I, rhIGF-II, rhGRO, rhEpo, rhMCP-1.
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human TNF soluble receptor II (TNF sRII), expressed in E. coli. This recombinant protein represents the non-glycosylated,N-terminal methionyl form of the naturally occuring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the transmembrane domain.

  • General notesEndotoxin level is < 10 ng per mg antibody as determined by the LAL method.

Properties

Applications

Our Abpromise guarantee covers the use of ab10502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at a concentration of 0.5 - 1 µg/ml, this is determined to detect recombinant human sTNF RII to a limit of 78 pg/well.
    WB: Use at a concentration of 1 - 2 µg/ml, this is determined using recombinant human sTNF RII at 5 ng/lane under non-reducing conditions. Predicted molecular weight: 75 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
    • Sequence similaritiesContains 4 TNFR-Cys repeats.
    • Post-translational
      modifications
      Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
      A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD120b antibody
      • Etanercept antibody
      • p75 antibody
      • p75 TNF receptor antibody
      • p75TNFR antibody
      • p80 TNF alpha receptor antibody
      • p80 TNF-alpha receptor antibody
      • Soluble TNFR1B variant 1 antibody
      • TBP-2 antibody
      • TBPII antibody
      • TNF R II antibody
      • TNF R2 antibody
      • TNF R75 antibody
      • TNF-R2 antibody
      • TNF-RII antibody
      • TNFBR antibody
      • TNFR-II antibody
      • TNFR1B antibody
      • TNFR2 antibody
      • TNFR80 antibody
      • TNFRSF1B antibody
      • TNR1B_HUMAN antibody
      • Tumor necrosis factor beta receptor antibody
      • Tumor necrosis factor binding protein 2 antibody
      • Tumor necrosis factor receptor 2 antibody
      • Tumor necrosis factor receptor superfamily member 1B antibody
      • Tumor necrosis factor receptor superfamily, member 1B antibody
      • Tumor necrosis factor receptor type II antibody
      • Tumor necrosis factor-binding protein 2 antibody
      see all

    References for Anti-Soluble TNF Receptor II antibody (ab10502)

    ab10502 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Hepatoma cells)
    Loading amount 10 µg
    Specification Hepatoma cells
    Treatment 2 ug TNFR2 for 24h
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Ms. Hyejeong Shin

    Verified customer

    Submitted Jul 09 2010

    Thank you for your enquiry. For both products, the immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"