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Product name
Anti-Soluble TNF Receptor II antibody
See all Soluble TNF Receptor II products (7) ...
Description
Goat polyclonal to Soluble TNF Receptor II
Specificity
By immunoblotting and ELISA, the antibody shows <15% cross-reactivity with recombinant human TNF-sRII. In addition, the antibody shows no cross-reactivity with other cytokines tested: rhACT II, rhANG, rhAnnexin V, rhAR, rhB7-1, rhB7-2, rmB7-2, rhBTC, rheta-NGF, rreta-NGF, rhBDNF, rmC10, rhCD8, rhCD28, rrCINC-1, rhCNTF, rhCNTF sRalpha, rrCNTF, rrCNTF sRalpha, rhEGF, rhENA-78, rhEotaxin, rmEotaxin, rhEPO, rhEPO R, rhFGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, rmFGF-8b, rhFGF-9, rhFlk2/Flt3 latent, rhG-CSF, rhG-CSF Ralpha, rmG-CSF, rhGDNF, rrGDNF, rhGM-CSF, rhGMCSF Ralpha, rmGM-CSF, rhGROalpha, rhGRObeta, rhGROgamma, rhHB-EGF, rhHCC-1, rhHRG-alpha, rhHGF, rhI-309, rhIFN-gamma, rmIFN-gamma, rrIFN- gamma, rhIGF-I, rhIGF-I R, rhIGF-II, rhIL-1alpha, rhIL-1 RI, rhIL-1 RII, rmIL-1alpha, rhIL-1beta, rmIL-1beta, rrIL-1beta, rhIL-1ra, rmIL-1ra, rhIL-2, rhIL-2 sRalpha, rhIL-2 sRbeta, rhIL-2 sRgamma, rmIL-2, rrIL-2, rhIL-3, rhIL-3 sRalpha, rmIL-3, rhIL-4, rhIL-4 sR, rmIL-4, rrIL-4, rhIL-5, rhIL-5 sRalpha, rmIL-5, rhIL-6, rhIL-6 sR, rmIL-6, rhIL-7, rhIL-7 R, rmIL-7, rhIL-8, rhIL-9, rhIL-9 sR, rmIL-9, rhIL-10, rhIL-10 sR, rmIL-10, rmIL-10 sR, rhIL-11, rmIL-11, rhIL-12, rmIL-12, rhIL-13, rmIL-13, rhIL-15, rhIL-17, rhIP-10, rmJAK-1, rmJAK-2, rmJE, rmKC, rhLIF, rhLIF R, rmLIF, rmLymphotactin, rhM-CSF, rmM-CSF, rhMCP-1, rhMCP-1 R, rhMCP-2, rhMCP-3, rhMidkine, rhMIF, rmMIG, rhMIP-1alpha, rmMIP-1alpha, rhMIP-1beta, rmMIP-1beta, rmMIP-2, rhMSP, rhNT-3, rhNT-4, rhOB, rmOB, rhOSM, rmOSM, rhPD-ECGF, hPDGF, pPDGF, rhPDGF-AA, rhPDGF-AB, rhPDGF-BB, rrPDGF-BB, rhPDGF Ralpha, rhPlGF, rhPTN, rhRANTES, rmRANTES, rhSCF, rmSCF, rhSDF-1beta/PBSF, rhsgp130, rhSLPI, rhSTAT-1, rhSTAT-2, rmSTAT-3, rmSTAT-4, hTfR, rhTGF alpha, rhTGF-beta1, rhTGF-beta2, rhTGF-beta3, raTGF-beta5, rhLAP (TGF-beta1), rhLatent TGF-beta1, rhTGF-beta sRII, rhTGF-B sRIII, rhTNF-alpha, rmTNF-alpha, rrTNF-alpha, rhTNF-beta, rhsTNF RI, rmsTNF RI, rhTPO, rmTPO, rhTyk-2, rhVEGF, rhVEGF/PlGF, rmVEGF.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Recombinant mouse TNF sRII, expressed in E. coli.
General notes
Endotoxin level is < 10 ng per mg antibody as determined by the LAL method.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody is purified using mouse TNF sRII affinity chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Growth Factors/Hormones >> TNF
Immunology >> Innate Immunity >> Cytokines >> Necrosis Factors
Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
Cell Biology >> Apoptosis >> Receptors >> Receptor Processing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
Application notes
ELISA: Use at a concentration of 0.5 - 1 µg/ml, this concentration is determined to detect recombinant mouse TNF sRII to a limit of 0.16 ng/well.
WB: Use at a concentration of 0.1 - 0.2 µg/ml, this concentration is determined using recombinant mouse TNF sRII at 5 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 75 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Sequence similarities
Contains 4 TNFR-Cys repeats.
Post-translational
modifications
Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP20333
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD120b antibody
- Etanercept antibody
- p75 antibody
see all
Database links
- Entrez Gene: 21938 Mouse
- SwissProt: P25119 Mouse
References for Anti-Soluble TNF Receptor II antibody (ab10504)
ab10504 has not yet been referenced specifically in any publications.
Publishing research using ab10504? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"