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Products:Neuroscience >> Neurotransmitter >> Neuropeptides >> Hormones
Overview
Product name
Anti-Somatostatin Receptor 1 antibody
See all Somatostatin Receptor 1 products (4) ...
Description
Rabbit polyclonal to Somatostatin Receptor 1
Specificity
This antibody reacts with a 60 kD protein, known as Somatostatin Receptor 1.
Tested applications
IHC-P, IHC-Fr, WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Rat)A synthetic peptide derived from the C-terminus of rat somatostatin receptor 1
Positive control
Pancreas
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA
Concentration
Concentration information loading...
Purity
Protein A purified
Primary antibody notes
The Somatostatin family of receptors have five members, sst1-sst5, as well as two isoforms of Somatostatin receptor 2, sst2A and sst2B. They are coupled to G protein and are involved in regulation of numerous processes including secretion of insulin, glucagons and growth hormone and excitation of neurons in central and peripheral nervous system.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neuroendocrinology >> GH Regulation
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> More GPCR
Neuroscience >> Neurotransmitter >> Neuropeptides >> Hormones
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/75.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr
IHC-Fr: 1/50 - 1/75.IHC-Fr: 1/50 - 1/75.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
ICC: Use a concentration of 1 µg/ml
Target
Function
Receptor for somatostatin with higher affinity for somatostatin-14 than -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and Na(+)/H(+) exchanger via pertussis toxin insensitive G proteins.
Tissue specificity
Fetal kidney, fetal liver, and adult pancreas, brain, lung, jejunum and stomach.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP30872
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- G-protein coupled receptor antibody
- Somatostatin R1 antibody
- Somatostatin receptor 1 antibody
see all
Database links
- Entrez Gene: 6751 Human
- Entrez Gene: 20605 Mouse
- Entrez Gene: 25033 Rat
- Omim: 182451 Human
- SwissProt: P30872 Human
- SwissProt: P30873 Mouse
- SwissProt: P28646 Rat
- Unigene: 248160 Human
see all
Anti-Somatostatin Receptor 1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Somatostatin Receptor 1 antibody (ab2366)
Formalin fixed paraffin embedded human pancreas stained with Somatostatin Receptor using ABC and AEC chromogen.
Western blot - Somatostatin Receptor 1 antibody (ab2366)
All lanes : Anti-Somatostatin Receptor 1 antibody (ab2366) at 1 µg/ml
Lane 1 : PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate (ab14882)
Lane 2 : Stomach (Human) Whole Cell Lysate - tumor tissue (ab29678)
Lane 3 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
Somatostatin Receptor 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunocytochemistry/ Immunofluorescence - Anti-Somatostatin Receptor 1 antibody (ab2366)
ICC/IF image of ab2366 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2366, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Somatostatin Receptor 1 antibody (ab2366)
This product has been referenced in:
- Fasciani Aet al. Overexpression and functional relevance of somatostatin receptor-1, -2, and -5 in endometrium and endometriotic lesions. J Clin Endocrinol Metab 95:5315-9 (2010). IHC-P; Human.Read more (PubMed: 20739383) »
See 1 publication for this product
Publishing research using ab2366? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"