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Overview

  • Product nameSonic Hedgehog Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 20 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 91.72 82% - 101%
    Serum 95.34 84% - 103%
    Plasma 76.43 68% - 89%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100639 Human Sonic Hedgehog (Sonic Hedgehog N Terminus) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of human Sonic Hedgehog in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for human Sonic Hedgehog coated on a 96-well plate. Standards and samples are pipetted into the wells and Sonic Hedgehog present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Sonic Hedgehog antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Sonic Hedgehog bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • RelevanceSonic Hedgehog, which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
    • Alternative names
        HHG 1HHG1HLP3
        HPE3MCOPCB5SHHSMMCISonic hedgehog homologSonic hedgehog proteinSonic hedgehog protein C productSonic hedgehog protein N productTPTTPTPS
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100639 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Sonic Hedgehog Human ELISA Kit images

      • ELISA - Sonic Hedgehog Human ELISA Kit (ab100639)
        ELISA - Sonic Hedgehog Human ELISA Kit (ab100639)

      Protocols

      References for Sonic Hedgehog Human ELISA Kit (ab100639)

      This product has been referenced in:
      • Nishimori H  et al. Prostate cancer cells and bone stromal cells mutually interact with each other through bone morphogenetic protein-mediated signals. J Biol Chem : (2012). Read more (PubMed: 22532569) »
      • Singh AP  et al. CXCL12/CXCR4 Protein Signaling Axis Induces Sonic Hedgehog Expression in Pancreatic Cancer Cells via Extracellular Regulated Kinase- and Akt Kinase-mediated Activation of Nuclear Factor ?B: IMPLICATIONS FOR BIDIRECTIONAL TUMOR-STROMAL INTERACTIONS. J Biol Chem 287:39115-24 (2012). Read more (PubMed: 22995914) »

      See all 3 Publications for this product

      Publishing research using ab100639 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100639 Submit a Question for ab100639

      Displaying 1 - 10 of 18 results for Abreviews and Q&A

      10
      Votes: Highest First

      Would ab100639 react with Indian Hedgehog?

      Thank you very much for your calls and for your patience while we have been in touch with the lab regarding your enquiry.

      Both the capture and detection antibodies are monoclonal, and as expected the epitopes have not been mapped so we don't kno...

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      What would be the more appropriate diluent to use for amniotic fluid?

      Thank you for contacting Abcam regarding ab100639.


      Assay Diluent B would be more appropriate if testing amniotic fluid (or any other body fluid other than serum or plasma).


      I hope this information is helpful. Please do not hesi...

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      I am currently writing the protocol for an ELISA of amniotic fluid samples with your product. I'm looking for advice on which diluent to use for the such a test. I cannot find anywhere what the constituents of either diluent are to help make my decision. ...

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      Thank you for contacting us. The constituents of the diluents are proprietary, but I am fairly certain you will want to use diluent C for amniotic fluid. I have asked the laboratory to confirm and will forward their reply.

      Thanks for looking into this. Did the lab offer a reason? If they are concerned that we will make our own ELISAs, feel free to price the antibodies such that it would be cost ineffective to do so. Again, ELISA is not our intent.

      Unfortunately, ...

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      Thank you for your reply.

      The antibody pair is considered intellectual property so they are not provided separately from the kits. Could you describe the exact assay that you would like to use the antibodies in?Ican ask the lab again and see if ...

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      Would like to order the antibodies separately.

      Thank you again for contacting us and for your patience.

      Unfortunately the lab is not able to provide these antibodies as components separate from the kit ab100639. I am very sorry for the inconvenience.

      We do have a pair of sonic hedg...

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      Would like to order the antibodies separately.

      Thank you very much fory our call yesterday and for your interest in these antibodies.

      I am still working on getting some more information about these antibodies, and I thank you for your patience. Our office will be closed tomorrow, but I will ...

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      What is the nature of the protein standard? Can you give me the aa sequence?

      Thank you for contacting Abcam regarding ab100639.


      We are not able to provide the exact amino acid sequence of this protein standard. This is the N-terminus amino acid sequence for the Sonic Hedgehog protein. I can tell you that the predic...

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      Good morning,

      I have purchased the Sonic Hedgehog Elisa kit ab100639, which works good in my first assay to evaluate Shh in the supernatant of cells in culture.
      However, the second and third time I have used it (from an opened and a new ki...

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      Thank you for your inquiry.

      As Assay Diluent B does contain proteins and other solutes it would not be abnormal to see a little cloudiness or precipitation, especially after being stored at a low temperature and/or for a long period of time. Read More

      Attached is the picture of SHH ELISA plate. In well A11 and A12, same sample was loaded in duplicate. Because this is an important sample, I also loaded another duplicate in well F3 and F4. But the color in well A12 showed great difference from others'. S...

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      Thank you for contacting Abcam.

      I have talked to the supplier about the issues you have been having and their response is below:

      High duplicate CV can be caused by many reasons but usually it is technique related. Below are the most co...

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      sELISA is still not working. Would like ELISA kit ab100639 as a replacement.

      Thank you for your call today, and I apologize that these antibodies still are not working.

      As we discussed, I'm sending a free of charge ELISA kit ab100639 on the order ***, which I expect to arrive by Friday.

      Please keep me updated a...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"