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ab50515 has been referenced in 2 publications.
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ab50515 staining Sonic Hedgehog in human Panc-1 cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed prior to blocking in 3% BSA for 30 minutes at 24°C. The primary antibody was used at 25 μg/ml and incubated with the sample for 16 hours at 4°C. Alexa fluor® 488 donkey polyclonal to rat Ig, diluted 1/700, was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Jennifer Dembinski
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