Sonic Hedgehog antibody [171018] (ab50515)

Product Name 

Sonic Hedgehog antibody [171018]

Product type 

Primary antibodies

Description 

Rat monoclonal [171018] to Sonic Hedgehog

Immunogen 

Purified, E. coli-derived, recombinant mouse sonic hedgehog N-terminal peptide (amino acids 25-198).

Reacts with 
(species key)

Hu, Ms

Specificity 

ab50515 recognizes N-terminal peptide. In immunoblotting, the antibody show approximately 50% crossreactivity with the N-terminal peptide from recombinant mouse Desert hedgehog (Dhh) (amino acids 23-198). The antibody shows no cross-reactivity with recombinant mouse Indian hedgehog (Ihh) (amino acid 66- 240) and no cross-reactivity with the C-terminal peptides of mouse Dhh (amino acids 199-396) or mouse Shh (amino acid 199-437).

Tested applications 
(see key)

ELISA, ICC/IF, IHC-FoFr, IHC-Fr, Neut, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 4 µg/ml.
ICC/IF: Use at a concentration of 25 µg/ml.
IHC-Fr: Use at a concentration of 25 µg/ml.
IHC-FoFr: 1/250 (PMID 18777197).
Neut: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Frozen mouse embryo sections.

Cellular localization 

Cell Membrane (N-terminal peptide) and Secreted (C-terminal peptide).

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Gut development >> Gut specification
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Developmental Biology >> Embryogenesis >> Morphogens
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> PTC & Wnt pathway
Stem Cells >> Signaling Pathways >> Hedgehog >> Secreted
Stem Cells >> Neural Stem Cells >> Surface Molecules
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other

Relevance 

Sonic Hedgehog, which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

171018

Isotype 

IgG2a

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: 5% Trehalose, PBS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

Endotoxin: < 0.1 EU (endotoxin units)/µg antibody as determined by the LAL (Limulus amebocyte lysate) method.

References for Sonic Hedgehog antibody [171018] (ab50515)

  This product has been used in:

Shen Y et al. Both cell-autonomous and cell non-autonomous functions of GAP-43 are required for normal patterning of the cerebellum in vivo. Cerebellum 7:451-66 (2008). IHC-FoFr; Mouse. PubMed: 18777197

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