Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
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ab21448 |
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ab21448 |
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ab63216 |
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ab75431 |
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ab82127 |
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ab95307 |
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ab123773 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab19897? Please let us know so that we can cite the reference in this datasheet
ab19897 has been referenced in 1 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Standard Curve for Sonic Hedgehog (Analyte:
All lanes : Anti-Sonic Hedgehog antibody (ab19897) at 1 µg/ml
Lane 1 : 22 kDa fragment of Mouse recombinant Sonic Hedgehog protein
Lane 2 : 22 kDa fragment of Mouse recombinant Sonic Hedgehog protein with
Lysates/proteins at 1 µg per lane.
Predicted band size : 48 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
ab19897 detects a 180 AA (22 kDa) recombinant fragment of mouse Sonic Hedgehog (lane 1). Binding of ab19897 to Sonic Hedgehog was reduced when blocking using the immunising peptide (lane 2).
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