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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
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Immunohistochemistry (PFA fixed) - SorCS1 antibody - Neuronal Marker (ab16641)
Immunofluorescent staining in rat hippocampus, using ab16641 SorCS1 antibody. [A] picture taken using a X10 objective and [B] X20 objective. Preincubation of antibody with the immunising peptide (ab16690) blocked the antibody from staining. ab16641 was used at 0.5µg/ml incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
Sophie Pezet, Univ London Kings Coll, United Kingdom
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SorCS1 antibody - Neuronal Marker (ab16641)
IHCP-image of SorCS1 staining using ab16641(1:500) on mouse Hippocampus sections. The tissue was fixed in formaldehyde and the epitope on sections were revealed using heat mediated antigen retrieval using citric Acid (pH 6). the sections were blocked using 15 BSA for 10 mins.
Carl Hobbs, King`s College London, United Kingdom
SorCS1 antibody - Neuronal Marker for Immunohistochemistry (PFA perfusion fixed frozen sections) in Mouse (16641)
SorCS1 antibody - Neuronal Marker for IHC-P in Mouse (16641)
SorCS1 antibody - Neuronal Marker for IHC-P in Rat (16641)
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