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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Overview
Product name
Anti-SorCS1 antibody - Neuronal Marker
See all SorCS1 products (2) ...
Description
Rabbit polyclonal to SorCS1 - Neuronal Marker
Tested applications
IHC (PFA fixed), IHC-P, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1100 to the C-terminus of Human SorCS1.
(Peptide available as ab16690.)
Positive control
rat brain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC (PFA fixed): Use a concentration of 0.5 µg/ml
IHC-P: 1/500Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-FoFr: 1/1000
Target
Relevance
SorCS1 belongs to the family of VPS10 domain-containing receptors (the name is derived from the yeast vacuolar protein sorting protein-10, which is involved in sorting of carboxy-peptidase Y from the Golgi apparatus to the vacuole). SorCS1 immunoreactivity is widespread in a population of neurons throughout the brain. Two different types of cellular localization were observed. Most SorCS1 immunoreactive neurons exhibit a punctate cytoplasmic staining which extends into the dendrites, whilst occassionally SorCS1 neuronal immunoreactivity is associated with the plasma membrane.
Cellular localization
Membrane; Single-pass type I membrane protein
Alternative names
- FLJ41758 antibody
- FLJ43475 antibody
- FLJ44957 antibody
see all
Database links
- Entrez Gene: 114815 Human
- Entrez Gene: 58178 Mouse
- Entrez Gene: 309533 Rat
- Omim: 606283 Human
- SwissProt: Q8WY21 Human
- SwissProt: Q8BQQ0 Mouse
- SwissProt: Q8BSV0 Mouse
- SwissProt: Q9JLC4 Mouse
see all
Anti-SorCS1 antibody - Neuronal Marker images:
Immunohistochemistry (PFA fixed) - SorCS1 antibody - Neuronal Marker (ab16641)
Immunofluorescent staining in rat hippocampus, using ab16641 SorCS1 antibody. [A] picture taken using a X10 objective and [B] X20 objective. Preincubation of antibody with the immunising peptide (ab16690) blocked the antibody from staining. ab16641 was used at 0.5µg/ml incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
Sophie Pezet, Univ London Kings Coll, United Kingdom
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SorCS1 antibody - Neuronal Marker (ab16641)
IHCP-image of SorCS1 staining using ab16641(1:500) on mouse Hippocampus sections. The tissue was fixed in formaldehyde and the epitope on sections were revealed using heat mediated antigen retrieval using citric Acid (pH 6). the sections were blocked using 15 BSA for 10 mins.
Carl Hobbs, King`s College London, United Kingdom
References for Anti-SorCS1 antibody - Neuronal Marker (ab16641)
ab16641 has not yet been referenced specifically in any publications.
Publishing research using ab16641? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"