Recombinant Anti-SorLA/SORL1 antibody [EPR14670] (ab190684)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14670] to SorLA/SORL1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SorLA/SORL1 antibody [EPR14670]
See all SorLA/SORL1 primary antibodies -
Description
Rabbit monoclonal [EPR14670] to SorLA/SORL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human cerebellum and SH-SY5Y cell lysates. Human cerebellum and Rat brain tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14670 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 248 kDa).
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 248 kDa). |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Likely to be a multifunctional endocytic receptor, that may be implicated in the uptake of lipoproteins and of proteases. Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. Binds the receptor-associated protein (RAP). Could play a role in cell-cell interaction. -
Tissue specificity
Expressed mainly in brain, where it is most abundant in the cerebellum, cerebral cortex and the occipital pole; low expression in the putamen and the thalamus. According to PubMed:9157966, found in spinal cord, testis, liver, kidney and pancreas with detectable levels in placenta, lung and heart. According to PubMed:8940146, expressed in the prostate, ovary, thyroid and spleen, but not found in kidney, liver, lung, skeletal muscle, bone marrow and adrenals. -
Sequence similarities
Belongs to the SORL1 family.
Contains 5 BNR repeats.
Contains 1 EGF-like domain.
Contains 6 fibronectin type-III domains.
Contains 11 LDL-receptor class A domains.
Contains 5 LDL-receptor class B repeats. -
Post-translational
modificationsThe propeptide removed in the N-terminus may be cleaved by furin or homologous proteases. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6653 Human
- Entrez Gene: 20660 Mouse
- Entrez Gene: 300652 Rat
- Omim: 602005 Human
- SwissProt: Q92673 Human
- SwissProt: O88307 Mouse
- SwissProt: Q9R0N2 Rat
- Unigene: 368592 Human
see all -
Alternative names
- C11orf32 antibody
- FLJ21930 antibody
- FLJ39258 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling SorLA/SORL1 with ab190684 at 1/1000 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling SorLA/SORL1 with ab190684 at 1/1000 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-SorLA/SORL1 antibody [EPR14670] (ab190684) at 1/5000 dilution + SH-SY5Y cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 248 kDa -
Anti-SorLA/SORL1 antibody [EPR14670] (ab190684) at 1/1000 dilution + Human cerebellum cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 248 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab190684 has been referenced in 8 publications.
- Alvarez-Mora MI et al. Heterozygous and Homozygous Variants in SORL1 Gene in Alzheimer's Disease Patients: Clinical, Neuroimaging and Neuropathological Findings. Int J Mol Sci 23:N/A (2022). PubMed: 35457051
- Jiang J et al. Upregulated of ANXA3, SORL1, and Neutrophils May Be Key Factors in the Progressionof Ankylosing Spondylitis. Front Immunol 13:861459 (2022). PubMed: 35464477
- Wei C et al. LncRNA NONMMUT055714 acts as the sponge of microRNA-7684-5p to protect against postoperative cognitive dysfunction. Aging (Albany NY) 13:12552-12564 (2021). PubMed: 33902009
- Hung C et al. SORL1 deficiency in human excitatory neurons causes APP-dependent defects in the endolysosome-autophagy network. Cell Rep 35:109259 (2021). PubMed: 34133918
- Knupp A et al. Depletion of the AD Risk Gene SORL1 Selectively Impairs Neuronal Endosomal Traffic Independent of Amyloidogenic APP Processing. Cell Rep 31:107719 (2020). PubMed: 32492427
- Jiang C et al. Association of Serum SorLA with Intimal Hyperplasia after Carotid Endarterectomy Operation: A Retrospective Analysis. Ann Vasc Surg 54:200-204 (2019). PubMed: 30103052
- Zollo A et al. Sortilin-Related Receptor Expression in Human Neural Stem Cells Derived from Alzheimer's Disease Patients Carrying the APOE Epsilon 4 Allele. Neural Plast 2017:1892612 (2017). WB, ICC/IF ; Human . PubMed: 28634550
- Thonberg H et al. Identification and description of three families with familial Alzheimer disease that segregate variants in the SORL1 gene. Acta Neuropathol Commun 5:43 (2017). PubMed: 28595629