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Overview

  • Product nameAnti-Spastin antibody [Sp 6C6]See all Spastin primary antibodies ...
  • Description
    Mouse monoclonal [Sp 6C6] to Spastin
  • Tested applicationsWB, ELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive controlTotal HeLa extract or rat brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab77144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    WB: 1/500. Detects a band of approximately 52 kDa (predicted molecular weight: 68 kDa). There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein. 52kDa is the major band.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceSpastin is thought have a role in microtubule dynamics through its function as a microtubule severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus.
    • Cellular localizationNucleus. Cytoplasm; perinuclear region.
    • Database links
      • Alternative names
          ADPSP antibodyFSP 2 antibodyFSP2 antibody
          KIAA1083 antibodySpast antibodySpastic paraplegia 4 antibodySPG 4 antibodySPG4 antibody
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      References for Anti-Spastin antibody [Sp 6C6] (ab77144)

      This product has been referenced in:

      See 1 Publication for this product

      Publishing research using ab77144 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab77144 Submit a Question for ab77144

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      more information about epitopes for ab77144 (Spastin antibody [Sp 6C6])?

      Thank you for having inquired about the epitope of ab77144 .

      I am very sorry for the long delay of my answer - i have been in contact with the laboratory as well to see whether there was any additional information. Unfortunately, we have no info...

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      Dear Sir or Madam,
      I´d like to contact you for some questions about the primary antibodies offered by your company:
      Anti-Spastin antibody [Sp 3G11/1] (ab31850)
      Anti-Spastin antibody [Sp 6C6] (ab77144)
      Could you please tell me, wh...

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      First of all I would like to apologise for the delay in getting back to you. I really appreciate your patience and comprehension.

      I contacted my lab colleagues and unfortunately there isn’t any information available about the epitope recog...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"