Anti-SPRY3 antibody (ab233424)
Key features and details
- Rabbit polyclonal to SPRY3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SPRY3 antibody -
Description
Rabbit polyclonal to SPRY3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human SPRY3 aa 50 to the C-terminus. N-terminal Tags. (Expressed in E.coli).
Database link: O43610 -
Positive control
- WB: Rat brain cerebrum tissue lysate - total protein (ab4033), Human lung lysate; Rat and mouse cerebrum lysate; Recombinant human SPRY3 protein. IHC-P: Human liver cancer, liver and kidney cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Relevance
Sprouty 3 (SPRY3) may function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. -
Cellular localization
Cytoplasmic. Membrane; Peripheral membrane protein. Note=Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor). -
Database links
- Entrez Gene: 10251 Human
- Entrez Gene: 236576 Mouse
- Entrez Gene: 498159 Rat
- Omim: 300531 Human
- SwissProt: O43610 Human
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Alternative names
- Antagonist of FGF signaling antibody
- HSPRY3 antibody
- Protein sprouty homolog 3 antibody
see all
Images
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Anti-SPRY3 antibody (ab233424) at 2 µg/ml + Rat cerebrum lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml -
Formalin-fixed, paraffin embedded human liver tissue stained for SPRY3 using ab233424 at 20 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin embedded human liver cancer tissue stained for SPRY3 using ab233424 at 20 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin embedded human renal cancer tissue stained for SPRY3 using ab233424 at 20 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Anti-SPRY3 antibody (ab233424) at 2 µg/ml + Mouse cerebrum lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml -
Anti-SPRY3 antibody (ab233424) at 3 µg/ml + Human lung lysate
Secondary
HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/ml -
Anti-SPRY3 antibody (ab233424) at 2 µg/ml + Recombinant human SPRY3 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab233424 has been referenced in 1 publication.
- Qiu B et al. Sprouty4 correlates with favorable prognosis in perihilar cholangiocarcinoma by blocking the FGFR-ERK signaling pathway and arresting the cell cycle. EBioMedicine 50:166-177 (2019). PubMed: 31761616