Overview
- Product nameAnti-Src antibodySee all Src primary antibodies ...
- DescriptionRabbit polyclonal to Src
- SpecificityThis antibody detects endogenous levels of total Src protein. It will react with Src (pY529), Yes (pY538), and Fyn (Y531).
- Tested applicationsICC/IF, WB, ELISA, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human, Pig
- Immunogen
Synthesized non-phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529.
- Positive controlHuman breast carcinoma tissue; HEK-293 cell extracts
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab47405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 1 - 5 µg/ml. |
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
| ELISA | ELISA: 1/10000. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
Target
- FunctionNon-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
- Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. - Post-translational
modificationsDephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
S-nitrosylation is important for activation of its kinase activity. - Cellular localizationCell membrane. Mitochondrion inner membrane.
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Database links
- Entrez Gene: 6714 Human
- Entrez Gene: 20779 Mouse
- Entrez Gene: 83805 Rat
- Omim: 190090 Human
- SwissProt: P12931 Human
- SwissProt: P05480 Mouse
- SwissProt: Q9WUD9 Rat
- Unigene: 195659 Human
- Unigene: 112600 Rat
see all
Target information above from: UniProt accession
P12931
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ASV antibodyAvian sarcoma virus antibodyc SRC antibody
- CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibodycSrc antibodyEC 2.7.10.2 antibodyNeuronal CSRC tyrosine specific protein kinase antibodyNeuronal SRC antibodyOncogene SRC antibodyOTTHUMP00000030931 antibodyOTTHUMP00000174476 antibodyOTTHUMP00000174477 antibodyp60 Src antibodyp60-Src antibodyp60Src antibodypp60c src antibodypp60c-src antibodypp60csrc antibodyProto oncogene tyrosine protein kinase Src antibodyProto-oncogene c-Src antibodyProto-oncogene tyrosine-protein kinase Src antibodyProtooncogene SRC antibodyprotooncogene SRC Rous sarcoma antibodySrc antibodySRC Oncogene antibodySRC_HUMAN antibodySRC1 antibodyTyrosine kinase pp60c src antibodyTyrosine protein kinase SRC 1 antibodyTyrosine protein kinase SRC1 antibodyv src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibodyV src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibodyv src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibodyvsrc avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
see all
Anti-Src antibody images
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Immunohistochemistry (Paraffin-embedded sections) - Src antibody (ab47405)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src antibody, in the presence and absence of blocking peptide. -
Western blot - Src antibody (ab47405)All lanes : Anti-Src antibody (ab47405) at 1/500 dilution
Lane 1 : HEK-293 cell extracts
Lane 2 : HEK-293 cell extracts with Blocking peptide
Predicted band size : 60 kDa
Observed band size : 60 kDa
Western blot analysis of extracts from HEK-293 cells using Src antibody at 1/500 in the presence and absence of blocking peptide. -
Immunocytochemistry/ Immunofluorescence - Src antibody (ab47405)ICC/IF image of ab47405 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47405, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Src antibody (ab47405)Anti-Src antibody (ab47405) at 1/1000 dilution + whole cell lysate prepared from mouse primary astrocytes at 15 µg
Secondary
Goat abti-rabbit IgG conjugated to HRP at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 60 kDa
Exposure time : 5 secondsThis image is courtesy of an anonymous abreview.
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Immunocytochemistry/ Immunofluorescence - Src antibody (ab47405)This image was kindly supplied by Dr Vladimir Milenkovic by Abreviewab47405 staining Src in human HEK-293 cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.5% Triton X-100 and then blocked using 5% serum for 20 minutes at 25°C. Samples were then incubated with primary antibody at 1/1000 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/5000 dilution. DAPI was used to stain the cell nuclei (blue). -
Immunocytochemistry/ Immunofluorescence - Src antibody (ab47405)This image is courtesy of an Abreview submitted by Dr. Vladimir Milenkovicab47405 staining Src in Pig retinal pigment epithelium primary cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in 0.1% TX100, 1% goat serum, 1XPBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/5000) was used as the secondary antibody. Nuclei were counterstained with DAPI.
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Western blot - Anti-Src antibody (ab47405)Anti-Src antibody (ab47405) at 1/500 dilution +Src protein (Active) (ab79635) at 0.001 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
References for Anti-Src antibody (ab47405)
This product has been referenced in:
- Sun Z et al. VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd. J Exp Med 209:1363-77 (2012). Read more (PubMed: 22689825) »
