Anti-Src (phospho S75) antibody (ab79308)
Key features and details
- Rabbit polyclonal to Src (phospho S75)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Src (phospho S75) antibody
See all Src primary antibodies -
Description
Rabbit polyclonal to Src (phospho S75) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Src (phospho S75).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 60 kDa.
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IHC-P | (1) |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 60 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsDephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
S-nitrosylation is important for activation of its kinase activity. -
Cellular localization
Cell membrane. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 6714 Human
- Entrez Gene: 20779 Mouse
- Entrez Gene: 83805 Rat
- Omim: 190090 Human
- SwissProt: P00523 Human
- SwissProt: P12931 Human
- SwissProt: P05480 Mouse
- SwissProt: Q9WUD9 Rat
see all -
Form
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
Alternative names
- ASV antibody
- Avian sarcoma virus antibody
- AW259666 antibody
see all
Images
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All lanes : Anti-Src (phospho S75) antibody (ab79308) at 1/1000 dilution
Lane 1 : extracts from COLO cells (treated
with EGF, 200ng/ml, 30mins)
Lane 2 : extracts from COLO cells (treated
with EGF, 200ng/ml, 30mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Left panel: immunohistochemistry analysis of paraffin-embedded Human brain tissue with ab79308 at 1/100 dilution. Right panel: same, after pre-incubation with the immunizing peptide
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ab79308 staining Src (phospho S75) in Dog osteosarcoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with acetone and blocked with 2% serum for 1 hour; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50 in serum) for 14 hours at 4°C. A Biotinylated Goat anti-rabbit IgG (1/400) was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab79308 has been referenced in 6 publications.
- Timmermans-Sprang E et al. P-cadherin mutations are associated with high basal Wnt activity and stemness in canine mammary tumor cell lines. Oncotarget 10:2930-2946 (2019). PubMed: 31105876
- Gong X et al. Expression of ILK in renal stroma is essential for multiple aspects of renal development. Am J Physiol Renal Physiol 315:F374-F385 (2018). PubMed: 29638158
- Kashiwagi K et al. A Ser75-to-Asp phospho-mimicking mutation in Src accelerates ageing-related loss of retinal ganglion cells in mice. Sci Rep 7:16779 (2017). PubMed: 29196663
- Winter M et al. Deciphering the Acute Cellular Phosphoproteome Response to Irradiation with X-rays, Protons and Carbon Ions. Mol Cell Proteomics 16:855-872 (2017). PubMed: 28302921
- Davis LE et al. A case study of personalized therapy for osteosarcoma. Pediatr Blood Cancer 60:1313-9 (2013). PubMed: 23526721
- Pan Q et al. Cdk5 targets active Src for ubiquitin-dependent degradation by phosphorylating Src(S75). Cell Mol Life Sci 68:3425-36 (2011). PubMed: 21442427