Recombinant Anti-Src (phospho Y419) antibody [EPR6239(2)] (ab133460)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6239(2)] to Src (phospho Y419)
- Suitable for: WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Src (phospho Y419) antibody [EPR6239(2)]
See all Src primary antibodies -
Description
Rabbit monoclonal [EPR6239(2)] to Src (phospho Y419) -
Host species
Rabbit -
Specificity
ab133460 is an antibody to Tyr419 (with Met). This is the equivalent as Tyr418 (without Met).
-
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- A431 cell lysate treated with Pervanadate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6239(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
Target
-
Function
Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsDephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
S-nitrosylation is important for activation of its kinase activity. -
Cellular localization
Cell membrane. Mitochondrion inner membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6714 Human
- Entrez Gene: 83805 Rat
- Omim: 190090 Human
- SwissProt: P00523 Human
- SwissProt: P12931 Human
- SwissProt: Q9WUD9 Rat
- Unigene: 195659 Human
- Unigene: 112600 Rat
-
Form
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
Alternative names
- ASV antibody
- Avian sarcoma virus antibody
- AW259666 antibody
see all
Images
-
All lanes : Anti-Src (phospho Y419) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution
Lane 1 : Untreated C6 (rat glioma) whole cell lysate
Lane 2 : C6 (rat glioma) treated with pervanadate whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDaPurified format.
Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
-
All lanes : Anti-Src (phospho Y419) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma) treated with pervanadate whole cell lysate
Lane 2 : Untreated HeLa (human cervix adenocarcinoma) treated with pervanadate whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDaPurified format.
Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
-
All lanes : Anti-Src (phospho Y419) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma) treated with pervanadate whole cell lysate
Lane 2 : Untreated A431 (human epidermoid carcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDaPurified format.
Blocking Buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM /TBST
-
All lanes : Anti-Src (phospho Y419) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution (undiluted)
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with Pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 60 kDa -
Undiluted format.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab133460 has been referenced in 4 publications.
- Zhu X et al. Ophiopogonin D suppresses TGF-ß1-mediated metastatic behavior of MDA-MB-231 breast carcinoma cells via regulating ITGB1/FAK/Src/AKT/ß-catenin/MMP-9 signaling axis. Toxicol In Vitro 69:104973 (2020). PubMed: 32818624
- Cochrane VA et al. Leptin modulates pancreatic ß-cell membrane potential through Src kinase-mediated phosphorylation of NMDA receptors. J Biol Chem 295:17281-17297 (2020). PubMed: 33037073
- Li X et al. The effects of c-Src kinase on EMT signaling pathway in human lens epithelial cells associated with lens diseases. BMC Ophthalmol 19:219 (2019). PubMed: 31703690
- Chen YC et al. 16-Hydroxycleroda-3,13-dien-15,16-olide induces anoikis in human renal cell carcinoma cells: involvement of focal adhesion disassembly and signaling. Onco Targets Ther 11:7679-7690 (2018). PubMed: 30464516