Anti-Stanniocalcin 1/STC antibody (ab229477)
Key features and details
- Rabbit polyclonal to Stanniocalcin 1/STC
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Stanniocalcin 1/STC antibody
See all Stanniocalcin 1/STC primary antibodies -
Description
Rabbit polyclonal to Stanniocalcin 1/STC -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey -
Immunogen
Recombinant fragment within Human Stanniocalcin 1/STC (internal sequence). The exact sequence is proprietary.
Database link: P52823 -
Positive control
- IHC-P: Mouse pancreas tissue. WB: Stanniocalcin 1/STC transfected HEK-293T whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
Target
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Function
Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia. -
Tissue specificity
Expressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, liver, spleen, peripheral blood leukocytes and adrenal medulla. -
Sequence similarities
Belongs to the stanniocalcin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 338078 Cow
- Entrez Gene: 6781 Human
- Entrez Gene: 20855 Mouse
- Entrez Gene: 81801 Rat
- Omim: 601185 Human
- SwissProt: Q9N0T1 Cow
- SwissProt: P52823 Human
- SwissProt: O55183 Mouse
see all -
Alternative names
- Stanniocalcin 1 antibody
- Stanniocalcin antibody
- Stanniocalcin-1 antibody
see all
Images
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Paraffin-embedded mouse pancreas tissue stained for Stanniocalcin 1/STC with ab229477 at a 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Stanniocalcin 1/STC antibody (ab229477) at 1/5000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : Stanniocalcin 1/STC transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 28 kDa12% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab229477 has been referenced in 8 publications.
- Chan KK et al. Stanniocalcin 1 is a serum biomarker and potential therapeutic target for HBV-associated liver fibrosis. J Pathol 257:227-238 (2022). PubMed: 35122667
- Lin F et al. Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer. J Exp Clin Cancer Res 41:129 (2022). PubMed: 35392966
- Zhao F et al. HIF-1α-regulated stanniocalcin-1 mediates gemcitabine resistance in pancreatic ductal adenocarcinoma via PI3K/AKT signaling pathway. Mol Carcinog 61:839-850 (2022). PubMed: 35785493
- Zhang C et al. Targeting POLE2 Creates a Novel Vulnerability in Renal Cell Carcinoma via Modulating Stanniocalcin 1. Front Cell Dev Biol 9:622344 (2021). PubMed: 33644060
- Peng F et al. Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. Cell Res 31:345-361 (2021). PubMed: 32859993
- Sun J et al. STC1 is a Novel Biomarker Associated with Immune Characteristics and Prognosis of Bladder Cancer. Int J Gen Med 14:5505-5516 (2021). PubMed: 34539184
- Qiu F et al. Development and Verification of an Immune-Based Gene Signature for Risk Stratification and Immunotherapeutic Efficacy Assessment in Gastric Cancer. Dis Markers 2021:4251763 (2021). PubMed: 34804261
- Xiong Y & Wang Q STC1 regulates glioblastoma migration and invasion via the TGF-ß/SMAD4 signaling pathway. Mol Med Rep 20:3055-3064 (2019). PubMed: 31432189