Anti-Staphylococcus Enterotoxin D antibody (HRP) (ab15926)
Overview
- Product nameAnti-Staphylococcus Enterotoxin D antibody (HRP)See all Staphylococcus Enterotoxin D primary antibodies ...
- DescriptionSheep polyclonal to Staphylococcus Enterotoxin D (HRP)
- ConjugationHRP
- SpecificityAbsorbance readings (410 nm) of less than 0.100 for 10 ng/ml preparations of other staphylococcal enterotoxins (of the B through E set), TSST, ET, and alpha hemolysin.
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with Staphylococcus enterotoxin D.
- Immunogen
Purified toxin (>95% pure).
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1% BSA, 0.01M PBS, pH 7.2 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab15926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| ELISA |
This is an approximate working dilution for use in a sandwich ELISA. This dilution will give an absorbance of >1.0 for a 10 ng standard after a 5 minute incubation.
WB: 1/1000. Predicted molecular weight: 31 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- RelevanceStaphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome. The illness is characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C (subtypes C1, C2, C3), D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. Enterotoxins are known to be most potent T cell mitogens. T cell activation accompanied by induction of interleukin 2 and interferon is conditioned by high affinity interaction of S.enterotoxins with class II main histocompatibility complex (MHC) molecules and subsequent presentation of the complex formed to a variable region of the T cell receptor.
- Cellular localizationSecreted
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Alternative names
- Ent D antibodyEntD antibodyEnterotoxin type D antibody
- SED antibodyStaphylococcal enterotoxin D antibodyStaphylococcus enterotoxin type D antibody
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References for Anti-Staphylococcus Enterotoxin D antibody (HRP) (ab15926)
ab15926 has not yet been referenced specifically in any publications.
