Products:Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Staphylococcus
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ab20920 has been referenced in 4 publications.
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ab20920 staining an abscess in rat calf tissue prepared as a 20 µm cryoslice. Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C. An Alexa Fluor® 568 goat anti-rabbit was used as the secondary
This image is courtesy of an Abreview submitted by Dr Dagmar Salber
ab20920 staining Staphylococcus aureus in human keratinocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol, permeabilized using 0.1% saponin/ PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab20920 at 10µg/ml for 14 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.Red: cadherinBlue: DAPIGreen: anti-Staphylococcus aureus (ab20920)
Image courtesy of an anonymous Abreview.
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