Anti-Staphylococcus aureus antibody (ab20920)

Dear Sir/Madam, Thank you for your information! I would like to try the antibody ab 20920 for immunoprecipitation. And then I can submit feedback to you in the form of an Ab-review. In my case, I will use to isolate S. aureus, so, it doesn't matter that these antibodies are polyclonal antibodies. You can send me these antibodies to my address (see below) and as soon as I have results I will contact you. Maybe you can send me one telephone number to discuss the things in between or e-mail address. Can you let me know when will be possible to get the product? Then I can plan all the experiments.

ANSWER:

Thank you very much for your message. If you have any questions, you can contact us under telephone 030 896 779 154 or via technical@abcam.com to discuss any issues or problems. I would be pleased to process the order for you to the given shipping address. In order to proceed with this, could you please confirm the billing address and provide a VAT number (otherwise, you would have to pay UK tax). Once we received this information, we can process the order and you will receive the antibody the next day, as we currently have it in stock. DISCOUNT CODE:xxxExpiration date: ddmm2012 I am very pleased to hear you would like to accept our offer and test ab20920 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

Dear Sir/Madam I found 2 possibilities but they are not indicated for immunoprecipitation. Do you think that the antibodies ab68954 or ab20920 can be used for this assay? Thank you in advance for your information.  

ANSWER:

Thank you for contacting us.

Indeed, neither ab68954 nor ab20920 have been tested for immunoprecipitation (IP) yet, but they could theoretically be used in this application. If it is just between the two, I would recommend ab20920 because it is an unconjugated antibody. The biotin-labeled antibody ab68954 might not bind to the Protein A or G agarose as well as the unconjugated antibody. Also, ab20920 seems to detect its antigen(s) in a rather native state (as it works with frozen tissue sections) which might be more suitable for IP.

However, these antibodies are polyclonal antibodies against various soluble and structural antigens of the whole Staphylococcus aureus. By using the antibodies in IP, a variety of proteins would be pulled down. If this is not appropriate for your experimental setting, I would be pleased to assist you in finding a suitable antibody (for example against a certain target protein).

If you decide to go ahead and purchase one of these products though, you might be eligible for our testing discount program. With this, you get a discount off a future purchase if you buy the antibody now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Telephone section Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint? A. Yes Q. Was it clear from the phone options which number you needed to press to get your issue resolved? A. I do not remember Protocol advice section Q. Did Abcam's Scientific Support give you protocol advice? A. No Q. How did you feel about being given protocol advice? A.   Q. Did the product work successfully after following the advice? A.   Q. Why have you not tried the protocol advice? (Select all that apply.) A. A. A. A. Free of charge replacement section Q. Did Abcam's Scientific Support give you a free of charge replacement? A. Yes  Q. Did the replacement product work successfully? A. No Credit note / refund section Q. Did Abcam's Scientific Support give you a credit note or a refund? A. No  Q. Would you have preferred a different outcome from receiving a credit note or refund? A.   Overall satisfaction section Q. How satisfied are you with how the complaint was handled? A. Unsatisfied Q. Could we have done anything to better resolve the problem? A. I was told that, since no one else complained about the lot number, it must be my fault. With no protocol advice or any real constructive criticism, I am left with no choice but to go with another antibody provider for my primary antibodies.  Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution? A. No, I would not mind Q. Please provide a phone number or email address where we can reach you. A. helen.m.davidson@gmail.com  Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products. A.  

ANSWER:

I have received a survey you have filled out relating your recent experience with ab20920. Reviewing my correspondance with you at the time I found that I had sent you a free replacement antibody to replace the faulty vial of ab20920 you received. I also offered to send you a complimentary free antibody. I am curious to know how the replacement vial I sent, ab68954, worked for you. In addition, you suggested waiting on the second free antibody until after you had tested ab68954. I would be happy to send you that second primary antibody at your convenience. I will be happy to help you resolve any remaining issues you man have with any of our products.

sorry for my impricise question. But you are right. We don't need information about how the antibodies are tested, but how you test your clarified antibodies. That's because we have problems by producing lysate from S. aureus. We used different lysis methods and had no positive signals with your antibodies. Propably we don't use the right one? It would be kind if you let us know how you produce lysate from S. aureus to get the best signal with your antibody (a protocol of your method would be perfect).

ANSWER:

Thank you for your enquiry.

This antibody has been tested for use by immunofluorescence. It is yet to be determined whether it is suitable for western blot analysis. Therefore we have not generated a lysate for immunoblotting analysis and do not have a corresponding protocol that we can provide you with. I am sorry that I cannot be of more help on this ocassion.

We do however have an antibody that HAS been shown to work by western blotting; Mouse monoclonal [332/423] to Methicillin Resistant Staphylococcus Aureus (ab35861). I am in touch with the source of this antibody to determine whether they can provide me with a protocol for its testing by western blotting. I appreciate your patience in this matter.

One more question please. Can you find out the method used for disrupting the bacteria. Was it mechanical or enzymatic and if so did they use lysostaphin and at what levels?

Thanks again

ANSWER:

The immunogen used to produce ab20920, Rabbit anti Staphylococcus aureus, is a mixture of intact organisms & soluble proteins from disrupted organisms. The cells were disrupted using a combination of sonication and freeze/thaw cycles. The immunogen preparation did not use any enzymatic methods.

Please contact us again if you have any additional questions.