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Products:Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Staphylococcus
Overview
Product name
Anti-Staphylococcus aureus antibody
See all Staphylococcus aureus products (7) ...
Description
Rabbit polyclonal to Staphylococcus aureus
Specificity
Soluble and structural antigens of the whole bacterium. Antiserum is not absorbed for related microorganisms.
Tested applications
ICC/IF, IHC-Frmore details
Cross reactivity
Reacts with
Staphylococcus aureus
Immunogen
Staphylococcus aureus strain ATCC 27660 (mixture of intact organisms and lysed cells).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.01M PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Toxin >> Bacterial Toxin >> Staphylococcus Toxin
Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Staphylococcus
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Immunohistochemistry (Frozen sections) - Staphylococcus aureus antibody (ab20920)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab20920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent concentration. (Use with acetone fixed tissues.)
Target
Relevance
Staphylococcus aureus are gram-positive bacteria that, although part of the normal flora of humans (nasal passages, skin and mucous membranes), have the potential to become opportunistic pathogens. S. aureus is a frequent cause of a wide range of infections and some bacterial strains have become resistant to many commonly used antibiotics.
Alternative names
- S. aureus antibody
Anti-Staphylococcus aureus antibody images:
Immunohistochemistry (Frozen sections) - Staphylococcus aureus antibody (ab20920)
ab20920 staining an abscess in rat calf tissue prepared as a 20 µm cryoslice. Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C. An Alexa Fluor® 568 goat anti-rabbit was used as the secondary
This image is courtesy of an Abreview submitted by Dr Dagmar Salber
References for Anti-Staphylococcus aureus antibody (ab20920)
This product has been referenced in:
- Mitchell Get al. Tomatidine Inhibits Replication of Staphylococcus aureus Small-Colony Variants in Cystic Fibrosis Airway Epithelial Cells. Antimicrob Agents Chemother 55:1937-45 (2011).Read more (PubMed: 21357296) »
- Mitchell Get al. Infection of Polarized Airway Epithelial Cells by Normal and Small-Colony Variant Strains of Staphylococcus aureus Is Increased in Cells with Abnormal Cystic Fibrosis Transmembrane Conductance Regulator Function and Is Influenced by NF-{kappa}B. Infect Immun 79:3541-51 (2011).Read more (PubMed: 21708986) »
See all 4 publications for this product
Publishing research using ab20920? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"