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Immunohistochemistry (Frozen sections) - Staphylococcus aureus antibody (ab20920)
Immunohistochemistry (Frozen sections) - Staphylococcus aureus antibody (ab20920)
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Product Name 

Staphylococcus aureus antibody

 

See all Staphylococcus aureus antibodies (7)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Staphylococcus aureus

Immunogen 

Staphylococcus aureus strain ATCC 27660 (mixture of intact organisms and lysed cells).

Reacts with 
(species key)

S aur

Specificity 

Soluble and structural antigens of the whole bacterium. Antiserum is not absorbed for related microorganisms.

Tested applications 
(see key)

ICC/IF, IHC-Fr


Abreviews 

Customer reviews
(see key)


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• ICC

1 Abreview

- average rating: 3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)

• ICC/IF

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• IHC-Fr

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)


IHC-Fr
(read Abreview)


ICC/IF
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent concentration. Use with acetone fixed tissues.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Microbiology >> Toxin >> Bacterial Toxin >> Staphylococcus Toxin
Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Staphylococcus

Relevance 

Staphylococcus aureus are gram-positive bacteria that, although part of the normal flora of humans (nasal passages, skin and mucous membranes), have the potential to become opportunistic pathogens. S. aureus is a frequent cause of a wide range of infections and some bacterial strains have become resistant to many commonly used antibiotics.

 

Staphylococcus aureus antibody (ab20920)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.01M PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

4.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Staphylococcus aureus antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Staphylococcus aureus antibody images, references, products related to ab20920 and other tools.

 

Staphylococcus aureus antibody images:

Product image  Immunohistochemistry (Frozen sections) - Staphylococcus aureus antibody (ab20920)

Immunohistochemistry (Frozen sections) - Staphylococcus aureus antibody (ab20920)

ab20920 staining an abscess in rat calf tissue prepared as a 20 µm cryoslice.  Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature.  The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C.  An Alexa Fluor® 568 goat anti-rabbit was used as the secondary

This image is courtesy of an Abreview submitted by Dr Dagmar Salber

See Abreview

 

References for Staphylococcus aureus antibody (ab20920)

Specific publication references   This product has been used in: (two most recent references)

Stenholm T et al. Methicillin-resistant Staphylococcus aureus screening by online immunometric monitoring of bacterial growth under selective pressure. Antimicrob Agents Chemother 53:5088-94 (2009). PubMed: 19752281

Salber D et al. Comparison of 99mTc- and 18F-ubiquicidin autoradiography to anti-Staphylococcus aureus immunofluorescence in rat muscle abscesses. J Nucl Med 49:995-9 (2008). PubMed: 18483088
If you publish research using ab20920 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Staphylococcus aureus

 

Staphylococcus aureus antibody - more information

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Staphylococcus aureus antibody - related products:

 

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Staphylococcus aureus antibody - other tools:

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