Abcam's Starch Assay Kit provides an easy, accurate assay to measure starch levels in a variety of samples. In the assay, starch is hydrolyzed to glucose which is oxidized to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay can detect starch at 0.0004 to 2 mg/ml.
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Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear a-1,4 linked polymer) and amylopectin, (highly a-1,6 branched a-1,4 polymer). Starch generally contains 0-25% amylose and 75-100% amylopectin.
Components | Identifier | 100 tests |
---|---|---|
Development Buffer | WM | 1 x 25ml |
Development Enzyme Mix (Lyophilised) | Green | 1 vial |
Hydrolysis Buffer | NM | 1 x 25ml |
Hydrolysis Enzyme Mix (Lyophilised) | Blue | 1 vial |
OxiRed Probe in DMSO | Red | 1 vial |
Starch Standard (2.0 mg/ml) | Yellow | 1 x 100µl |
Our Abpromise guarantee covers the use of ab83393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Functional Studies | Use at an assay dependent dilution. |
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"