Directly conjugated antibodies for immunofluorescence

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Customer reviews and FAQs

Overview

  • Product nameStarch Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 4 µg/ml
  • Range
    4 µg/ml - 2000 µg/ml
  • Assay time
    0h 40m
  • Product overview

    Abcam's Starch Assay Kit provides an easy, accurate assay to measure starch levels in a variety of samples. In the assay, starch is hydrolyzed to glucose which is oxidized to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay can detect starch at 0.0004 to 2 mg/ml.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear α-1,4 linked polymer) and amylopectin, (highly α-1,6 branched α-1,4 polymer). Starch generally contains 0-25% amylose and 75-100% amylopectin.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceStarch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear alpha-1,4 linked polymer) and amylopectin, (highly alpha-1,6 branched alpha-1,4 polymer). Starch generally contains 0-25% amylose and 75–100% amylopectin.
  • Alternative names
    • Amylopectin
    • Amylose

Applications

Our Abpromise guarantee covers the use of ab83393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Starch Assay Kit images

  • Functional Studies - Starch Assay Kit (ab83393)
    Functional Studies - Starch Assay Kit (ab83393)
    Starch Standard Curve: Different types of pure starch were extracted with 10N KOH/H3PO4 as described. Assays were performed following the kit protocol.

Protocols

References for Starch Assay Kit (ab83393)

This product has been referenced in:
  • Mittal A  et al. TOPOISOMERASE 6B is involved in chromatin remodelling associated with control of carbon partitioning into secondary metabolites and cell walls, and epidermal morphogenesis in Arabidopsis. J Exp Bot N/A:N/A (2014). Functional Studies . Read more (PubMed: 24821950) »
  • Martin AR & Thomas SC Size-dependent changes in leaf and wood chemical traits in two Caribbean rainforest trees. Tree Physiol 33:1338-53 (2013). Read more (PubMed: 24336517) »

See all 2 Publications for this product

Publishing research using ab83393 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an abreview for ab83393 Submit a question for ab83393

6 Abreviews or Q&A

10
Votes: Highest First


Our customer has responded:

‘All in all, I have not detected enough amount of starch from both the soluble and insoluble supernatants.



So, I think I’m having issues with the starch extraction protocol....

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Thank you for your response.

My colleague is out of office for a couple of days this week and she has asked me to look after her customers during her absence.

I am very sorry that your customer is having a hard time extracting starc...

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Hi,

The customer has responded regarding this starch assay kit:

;Now the measurement of resistant starch seemed to work alright and I 've checked it against a few plant species. The recent trials, I used DMSO rather than 10M KOH (...

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Thank you very much for that feedback. This sort of information is indeed very important for your customers.

I would strongly encourage your customer to provide us with an Abreview for the product. This would simply involve sharing the protoc...

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Our customer has responded to your questions:

1. Have you performed the standard curve? Has this produced the expected graph? What were your readings?

I performed a standard curve, but I am not sure whether this has produced the e...

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Thank you once again for your enquiries which have been forwarded to me as my colleague Karin is currently away from the office.

Karin has discussed this withour source for this antibody, and they have kindly provided the following advise: <...

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Hi,

A researcher has contacted us in regards to this product: Starch Assay [Ab83393]

“ I am using the starch assay kit to measure soluble starch levels in Medicago seeds. I am measuring the starch content, via the colorimetr...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In ...

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Our customer would like to inquire about the Starch Assay Kit (ab83393). The sample he would like to test is the juveniles and leaves of Arabidopsis thaliana, but he is not sure whether ab83393 is suitable. And if it is suitable for Arabidopsis thalian...

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Thank you for contacting us.

Theoretically this kit can be used with any type of starch containing samples. I assume it can detect Starch inleaves of Arabidopsis thaliana also. Please be advised that we haven't used this kit for detection of...

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Is this http://www.abcam.com/Starch-Assay-Kit-ab83393.html suitable to use for plant (legume) seeds?



Kind regards,

Thank you for contacting us.

Theoretically this kit can be used with any type of starch containing samples. I assume it can detect Starch in legume seeds also. please be advised that we haven't used this kit for detection of starch in legume...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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