Anti-Stargazin antibody [N245/36] (ab167445)
Key features and details
- Mouse monoclonal [N245/36] to Stargazin
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
-
Product name
Anti-Stargazin antibody [N245/36]
See all Stargazin primary antibodies -
Description
Mouse monoclonal [N245/36] to Stargazin -
Host species
Mouse -
Specificity
ab167445 does not cross-react with Stargazin 3. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Fusion protein corresponding to Rat Stargazin aa 200 to the C-terminus (C terminal).
Database link: Q71RJ2 -
Positive control
- WB: Rat brain tissue lysate. ICC/IF: SK-N-BE cells.
-
General notes
The clone number has been updated from S245-36 to N245/36, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N245/36 -
Isotype
IgG2a -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 36 kDa.
|
|
ICC/IF |
1/100.
|
Notes |
---|
WB
1/1000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/100. |
Target
-
Function
Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily. -
Post-translational
modificationsPhosphorylation of Thr-321 impairs interaction with DLG1 and DLG4. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 10369 Human
- Entrez Gene: 84347 Rat
- Omim: 602911 Human
- SwissProt: Q9Y698 Human
- SwissProt: Q71RJ2 Rat
- Unigene: 146766 Human
- Unigene: 72939 Rat
-
Alternative names
- AW060990 antibody
- B230105C07Rik antibody
- B930041E13Rik antibody
see all
Images
-
4% formaldehyde-fixed SK-N-BE cells labeling Stargazin using ab167445 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
-
Lane 1 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/100 dilution
Lane 2 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/250 dilution
Lane 3 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/500 dilution
Lane 4 : Anti-Stargazin antibody [N245/36] (ab167445) at 1/1000 dilution
All lanes : Rat brain lysate
Predicted band size: 36 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab167445 has not yet been referenced specifically in any publications.