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StayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible) (ab103743)

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Overview

  • Product nameStayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible)
  • General notesStayBlue/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. StayBlue/AP produces a vibrant blue color. It is insoluble in alcohol and xylene solutions (both aliphatic and hydrocarbon based) therefore sections can be dehydrated in alcohol, cleared in xylene substitute and permenently mounted. 30ml clear StayBlue/AP substrate buffer. 1ml concentrated StayBlue/AP chromagen 1 empty mixing dropping bottle Working Solution Aliquot 3ml of StayBlue/AP substrate buffer in a mixing bottle. Add 20µl (one drop) of concentrated StayBlue/AP chromogen. Replace tip, mix and allow the solution to reach room temperature before using. Note: The working chromagen-substrate solution should be prepared fresh and used withing 25 mins of preparation. Any solution not used during this peeriod should be discarded.
    Protocol
    • After SA-AP or AP-polymer incubation incubation, wash tissue sections with wash buffer.
    • Wipe slides removing excess buffer. Add enough drops of StayBlue/AP working solution to cover tissue sections.
    • Incubate for 5-25 minutes at room temperature. For optimal results, observe reaction under the microscope for signal developmant. Once the desired signal to noise ratio is acheived, stop the reaction by rinsing the slides with DI H2O. Note: Increasing incubation temperature to 37°C will increase sensitivity and decrease needed incubation time.
    • Counterstain. Nuclear Fast Red prvides good contrast. Wash with DI H2O.
    • Dehydrate sections in alcohol, clear xylene substitute and mount in permenant mounting medium. Note: Use increasing concentrations of ethanol (up to 100%) to dehydrate. Use xylene substitute instead of xylene.
    • Alternatively, slides can be air dried. After rinsing off counterstain in DI H2O, leave the slides on the benchtop for at least 20 minutes to air dry, then permanently mount.

    • Properties

  • Storage instructionsStore at +4°C.
  • Concentration information loading...
  • Research Areas

    • StayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible) images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - StayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible) (ab103743)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - StayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible) (ab103743)
      StayBlue/AP staining CD20 in Human tonsil tissue.

    References for StayBlue/AP 30ml (Alcohol and Xylene Substitute Compatible) (ab103743)

    ab103743 has not yet been referenced specifically in any publications.

    Publishing research using ab103743 ? Please let us know so that we can cite the reference in this datasheet

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    Inquiry: Hi there, I was wondering whether you can help. I have to stain mouse tissue using an unconjugated primary antibody (Anti-Mouse/Rat MULT1, Host - Armenian Hamster IgG) and a biotinylated secondary one (Anti-Armenian Hamster IgG Biotin, Host - Goa...

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    Thank you for your inquiry.

    I can suggest to use a strepavidin that is coupled to an enzyme, for example: ab128985 (Streptavidin Peroxidase (Ready-To-Use)) and combine this with a suitable chromogen, for example: ab94665 (DAB substrate kit)
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    Hello,
    I am interested in your product StayGreen and StayRed substrates for immunohistochemistry. I am currently planning to do double immunohistochemistry and are looking into what colors that would be good together. To combine with a DAB staining wo...

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    The source of StayRed and StayBlue recommend to do sequential staining. Their recommendations on chromogens for double staining are DAB and StayRed (http://www.abcam.com/ab103741). In order to use these chromogens first use DAB, then RED, followed by...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"