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Question (22504) | StayGreen/AP (Alcohol and Xylene Substitute Compatible) (ab103745)

Could the StayRed and StayGreen chromegen's be used on the same tissue as a sort on non-fluorescent double-labelling? Thanks! Amanda

Thank you for your enquiry. I can confirm that these products can be used together for double staining. However, I can recommend to consider the following: 1. We would suggest to ensure the primary antibodies used in the stains undergo the same conditions for antigen/epitope retrieval. 2. Since the StayRed is a permanent Chromogen (i.e. both alcohol and xylene insoluble) it must be used as the first Chromogen in the double staining protocol. 3. The StayGreen is also alcohol insoluble, however since it is soluble in xylene it must be used with a xylene substitute. The tissue can then be mounted permanently. I was hoping to obtain any images of double staining for you, but regrettably our source does not have these available. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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