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Anti-Stella antibody - Primordial Germ Cell Marker (ab19878)

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1 question for ab19878

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Question 1

Wednesday 17-May-2006

Can you tell me more about this antibody? Does it work in IHC and WB, or not?

ANSWER:

  

Thank you for your enquiry.

I have discussed this antibody with the scientist involved in characterizing it. The antibody recognized a band of 37 kDa in mouse embryonic germ cell lysate. This band was not blocked by the peptide and the correct size band was not observed. It is possible, however, that the expression level is so low in the lysate that the protein cannot be detected. It may work in Western blot if the correct lysate can be obtained, however, we cannot guarantee it for that application.

We are selling the antibody because of the excellent IHC image. The staining with the antibody is observed only in mouse embryonic genital ridges (E14.5) and only in Oct4-positive cells, an expression pattern consistent with the expected localization of this protein. We believe it is unlikely that this antibody would happen to recognize the immunizing peptide in ELISA and a protein with the expected staining pattern in IHC, and not recognize the Stella protein. Without a knockout line, of course we cannot definitively prove this, but this would be no different for any antibody where the knockout is not available.

In short, we cannot guarantee the antibody in Western blot at the moment, but we are confident that it is detecting Stella in IHC based on the beautiful IHC image we have obtained.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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