Recombinant Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] (ab168380)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11695(B)] to Steroidogenic Factor 1/SF-1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)]
See all Steroidogenic Factor 1/SF-1 primary antibodies -
Description
Rabbit monoclonal [EPR11695(B)] to Steroidogenic Factor 1/SF-1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Steroidogenic Factor 1/SF-1.
Database link: Q13285 -
Positive control
- Rat and mouse brain, Jurkat and HepG2 whole cell lysate (ab7900).
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11695(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168380 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
Target
-
Function
Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. -
Involvement in disease
Defects in NR5A1 are a cause of 46,XY disorder of sex development (46,XY DSD) [MIM:612965]; also known as XY sex reversal with or without adrenal failure. A congenital condition in which development of chromosomal, gonadal, or antomic sex is atypical. 46,XY DSD is a disorder of gonadal (testicular) development, which may be complete or partial. The complete form includes streak gonads, normal mullerian structures, and normal female external genitalia. The partial form includes ambiguous external genitalia and partial development of mullerian and wolffian structures.
Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect (ACIWOD) [MIM:184757]. ACIWOD is characterized by severe 'slackness' muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH.
Defects in NR5A1 are the cause of premature ovarian failure type 7 (POF7) [MIM:612964]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR5 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Post-translational
modificationsAcetylation stimulates the transcriptional activity. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 2516 Human
- Entrez Gene: 26423 Mouse
- Entrez Gene: 83826 Rat
- Omim: 184757 Human
- SwissProt: Q13285 Human
- SwissProt: P33242 Mouse
- SwissProt: P50569 Rat
- Unigene: 495108 Human
see all -
Alternative names
- AD4BP antibody
- Adrenal 4 binding protein antibody
- Adrenal 4-binding protein antibody
see all
Images
-
All lanes : Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] (ab168380) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 52 kDa -
All lanes : Anti-Steroidogenic Factor 1/SF-1 antibody [EPR11695(B)] (ab168380) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (7)
ab168380 has been referenced in 7 publications.
- Onizuka H et al. Metabolic Reprogramming Drives Pituitary Tumor Growth through Epigenetic Regulation of TERT. Acta Histochem Cytochem 54:87-96 (2021). PubMed: 34276102
- Blesson CS et al. In utero low-protein-diet-programmed type 2 diabetes in adult offspring is mediated by sex hormones in rats†. Biol Reprod 103:1110-1120 (2020). PubMed: 32766739
- Idrees M et al. SHP2 Nuclear/Cytoplasmic Trafficking in Granulosa Cells Is Essential for Oocyte Meiotic Resumption and Maturation. Front Cell Dev Biol 8:611503 (2020). PubMed: 33553147
- Prasad SK et al. Radioprotective effect of ethanolic extract of Alocasia indica on ?-irradiation-induced reproductive alterations in ovary and uterus. Int J Radiat Biol N/A:1-14 (2019). PubMed: 31314632
- Huang H et al. CRISPR/dCas9-mediated activation of multiple endogenous target genes directly converts human foreskin fibroblasts into Leydig-like cells. J Cell Mol Med 23:6072-6084 (2019). PubMed: 31264792
- Idrees M et al. PTPN11 (SHP2) Is Indispensable for Growth Factors and Cytokine Signal Transduction During Bovine Oocyte Maturation and Blastocyst Development. Cells 8:N/A (2019). PubMed: 31635340
- Medwid S et al. Prenatal exposure to bisphenol A disrupts adrenal steroidogenesis in adult mouse offspring. Environ Toxicol Pharmacol 43:203-8 (2016). WB ; Mouse . PubMed: 27017381